SMPD3/NSMase2 Antibody

Western blot shows lysates of RPMI 8226 human multiple myeloma cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SMPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7184) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for SMPD3 at approximately 70 to 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SMPD3/NSMase2 Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human SMPD3
  Val2-Ala655
  Accession # Q9NY59
• Specificity: Detects human SMPD3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SMPD3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SMPD3/NSMase2 Antibody

• EC 3.1.4.12
• FLJ22593
• MGC138443
• Neutral sphingomyelinase 2
• Neutral sphingomyelinase II
• NSMASE2
• nSMase-2
• SMPD3
• sphingomyelin phosphodiesterase 3
• sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinaseII)

Background

SMPD3 (Sphingomyelin phosphodiesterase 3; also Neutral sphingomyelinase 2/nSMase2) is a 69-74 kDa member of the neutral sphingomyelinase family of enzymes. It is a monomeric Golgi/plasma membrane enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide and phosphorylcholine. This generates second messenger components that participate in signal transduction. Human SMPD3 is a two transmembrane, 655 amino acids molecule. It contains an N-terminal luminal segment (aa 1-10), a cytoplasmic region (aa 32-64), and one catalytic domain (aa 340-646). Phosphorylation increases its MW to 78 kDa in SDS-PAGE. There is one potential isoform that possesses an Asn substitution for aa 569-587. Over aa 2-655, human SMPD3 shares 91% aa sequence identity with mouse SMPD3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMPD3/NSMase2 Antibody (AF7184)(1)

Publication using AF7184

• L Perreault, SA Newsom, A Strauss, A Kerege, DE Kahn, KA Harrison, JK Snell-Berg, T Nemkov, A D'Alessand, MR Jackman, PS MacLean, BC Bergman Intracellular localization of diacylglycerols and sphingolipids influences insulin sensitivity and mitochondrial function in human skeletal muscle JCI Insight, 2018-02-08;3(3):. 2018-02-08 [PMID: 29415895] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SMPD3
• Uniprot:
  • Q9NY59(Human)