SMG7 Antibody

Western Blot: SMG7 Antibody [NBP1-22967] - Western Blot and Immunoprecipitation: by Western Blot : Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. SMG7 was also immunoprecipitated byanother rabbit anti-SMG7 antibody.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SMG7 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1087 and 1137 of human suppressor with morphogenetic effect on genitalia protein 7 (ever shorter telomeres 1C) using the numbering given in entry NP_775179.1 (GeneID 9887).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMG7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 5-15 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMG7 Antibody

• breast cancer-associated antigen SGA-56M
• C1orf16
• chromosome 1 open reading frame 16
• EST1 telomerase component homolog C
• EST1CKIAA0250SGA56M
• EST1-like protein C
• ever shorter telomeres 1C
• FLJ23717
• hSMG-7
• protein SMG7
• SMG-7 homolog
• Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
• SMG-7

Background

SMG7 plays a role in the dephosphorylation of UPF1, an RNA-dependent ATPase and helicase critical to nonsense-mediated mRNA decay (NMD). It is a homolog of the C. elegans NMD factor Smg7 (suppressor with morphogenetic effect on genitalia protein 7). SMG7 functions as an adaptor to target protein phosphatase 2A to UPF1 for dephosphorylation of UPF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMG7 Antibody (NBP1-22967)(2)

Publications using NBP1-22967

• Lykke-Andersen S, Chen Y, Ardal BR et al. Human nonsense-mediated RNA decay initiates widely by endonucleolysis and targets snoRNA host genes. Genes Dev. 2014-11-15 [PMID: 25403180] (WB, Human)
  
  Details:
  SMG7 antibody used for WB analyses of proteins coimmunoprecipitated with endogenous UPF1 from the HEK293-beta-39 cell line (Figure 2G).
• Sakaki K, Yoshina S, Shen X, Han J, Desantis MR, Xiong M, Mitani S, Kaufman RJ. RNA surveillance is required for endoplasmic reticulum homeostasis. Proc Natl Acad Sci U S A;109(21):8079-84. 2012-05-22 [PMID: 22562797] (WB, Human)

Bioinformatics

• Gene Symbol: SMG7
• Entrez:
  • 9887(Human)
• Uniprot:
  • NP_775179.1(Human)