SMEK2 Antibody

Images

 
Western Blot: SMEK2 Antibody [NB100-2881] - Detection of Human and Mouse PPP4R3 Beta on HeLa whole cell lysate using NB100-2881. PPP4R3 beta was also immunoprecipitated by rabbit anti-PPP4R3 beta antibody NB100-2880.
Immunohistochemistry-Paraffin: SMEK2 Antibody [NB100-2881] - : FFPE section of human prostate carcinoma.
Immunohistochemistry-Paraffin: SMEK2 Antibody [NB100-2881] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PPP4R3 Beta used at a dilution of 1:250.
Immunohistochemistry-Paraffin: SMEK2 Antibody [NB100-2881] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PPP4R3 Beta used at a dilution of 1:100.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

SMEK2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 715 and the C-terminus (residue 764) of human Protein Phosphatase 4, Regulatory Subunit 3 Beta (KIAA1387) using the numbering given in entry NP_065196.1 (GeneID 57223).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMEK2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SMEK2 Antibody

  • FLFL2
  • FLJ31474
  • KIAA1387serine/threonine-protein phosphatase 4 regulatory subunit 3B
  • PP4R3B
  • PPP4R3B
  • PSY2
  • SMEK homolog 2
  • SMEK homolog 2, suppressor of mek1 (Dictyostelium)
  • smk1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMEK2
Entrez
Uniprot