SMARCC1 Antibody (JB43-43)

Western Blot: SMARCC1 Antibody (JB43-43) [NBP2-75666] - Analysis of SMARCC1 on different lysates with Rabbit anti-SMARCC1 antibody at 1/500 dilution.Lane 1: HL-60 cell lysate (10 ug/Lane)Lane 2: Mouse thymus tissue lysate (20 ug/Lane)Predicted band size: 123 kDaObserved band size: 155 kDaExposure time: 1 minute;6% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: SMARCC1 Antibody (JB43-43) [NBP2-75666] - Staining SMARCC1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SMARCC1 Antibody (JB43-43) [NBP2-75666] - Analysis of paraffin-embedded human small intestine tissue using anti-SMARCC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: SMARCC1 Antibody (JB43-43) [NBP2-75666] - Analysis of K562 cells with SMARCC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Western Blot: SMARCC1 Antibody (JB43-43) [NBP2-75666] - Analysis of SMARCC1 on different cell lysates using anti-SMARCC1 at 1/500 dilution.Positive control:Line 1: Mouse thymus tissue Line 2: HL-60

Immunohistochemistry-Paraffin: SMARCC1 Antibody (JB43-43) [NBP2-75666] - Analysis of paraffin-embedded mouse testis tissue using anti-SMARCC1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SMARCC1 Antibody (JB43-43) [NBP2-75666] - Analysis of paraffin-embedded human lymph node tissue using anti-SMARCC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: SMARCC1 Antibody (JB43-43) [NBP2-75666] - Analysis of paraffin-embedded human esophagus tissue using anti-SMARCC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

SMARCC1 Antibody (JB43-43) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human SMARCC1 aa 680-820 / 1,105. (SwissProt: Q92922 Human; SwissProt: D3ZJU5 Rat)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SMARCC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:1000
• Theoretical MW: 123 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SMARCC1 Antibody (JB43-43)

• BAF155SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily C member 1
• BRG1-associated factor 155
• chromatin remodeling complex BAF155 subunit
• CRACC1
• mammalian chromatin remodeling complex BRG1-associated factor 155
• Rsc8
• SRG3
• subfamily c, member 1
• SWI/SNF complex 155 kDa subunit
• SWI/SNF complex subunit SMARCC1
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI3

Background

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCC1/BAF155 is part of the BAF complex that associates with SMARCA2/Brm and SMARCA4/Brg. The mouse homolog is Srg3 and has been found to be required for embryogenesis and brain development. SMARCC1 is also identified as SWI/SNF complex 155 kDa subunit, and BRG1-associated factor 155.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMARCC1