SLC7A5/LAT1 Antibody

Images

 
Western Blot: SLC7A5 Antibody [NBP1-51935] (0.3ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

SLC7A5/LAT1 Antibody Summary

Immunogen
Peptide with sequence C-RHRKPELERPIKVN corresponding to internal region according to NP_003477.4.
Epitope
C-RHRKPELERPIKVN
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SLC7A5
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 60 kDa band observed in human spleen lysates (calculated MW of 55.0 kDa band according to NP_003477.4). Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than WB.
Publications
Read Publication using NBP1-51935.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SLC7A5/LAT1 Antibody

  • 4F2 LC
  • 4F2LC
  • CD98
  • CD98lc
  • D16S469EL-type amino acid transporter 1
  • E16
  • Integral membrane protein E16
  • large neutral amino acids transporter 1
  • large neutral amino acids transporter small subunit 1
  • LAT1
  • LAT1hLAT1
  • MPE16
  • MPE16CD98 light chain
  • SLC7A5
  • sodium-independent neutral amino acid transporter LAT1,4F2LC
  • solute carrier family 7 (cationic amino acid transporter, y+ system), member 5,4F2 light chain
  • Solute carrier family 7 member 5
  • TA1
  • y+ system cationic amino acid transporter

Background

Mammalian cells have several amino acid transport systems. These systems have been defined by both activity and specific genes. L-type amino acid transporter 1 (LAT1) is a 12 membrane-spanning protein. LAT1 is a Na + -independent neutral amino acid transporter agency and essential for the transporter of large neutral amino acid such as Leucine, Isoleucine, and Valine through the plasma membrane. LAT1 has been proposed to be one of the major nutrient transport systems at the blood-brain barrier. Drugs such as L Dopa are transported by LAT1. LAT1 is thought to be up-regulated to support the high protein synthesis for cell growth in some tumor cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLC7A5/LAT1 Antibody (NBP1-51935)(1)

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLC7A5
Entrez
Uniprot