SLC39A4/ZIP4 Antibody [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

SLC39A4/ZIP4 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SLC39A4
  Met1-Tyr337
  Accession # Q78IQ7
• Specificity: Detects mouse SLC39A4 in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLC39A4/ZIP4 Antibody [Alexa Fluor® 488]

• acrodermatitis enteropathica, zinc-deficiency type
• AEZ
• FLJ20327
• MGC74741
• SLC39A4
• solute carrier family 39 (zinc transporter), member 4
• Solute carrier family 39 member 4
• zinc transporter ZIP4
• ZIP4
• ZIP-4
• ZIP4AEZ
• Zrt- and Irt-like protein 4

Background

SLC39A4 (Solute carrier family 39 member 4; also Zip-4) is a 75-90 kDa member of the ZIP transporter family of proteins. It is expressed by select cell types, including pancreatic beta -cells plus epithelium of the choroid plexus and intestine. SLC39A4 is part of a zinc-sensitive cell cycling system. When extracellular zinc levels are high, SLC39A4 is generally found internally associated with endosomes, and is unavailable at the cell membrane to transport zinc into the cell. Under low zinc conditions, SLC39A4 generally appears in the plasma membrane to ensure adequate zinc uptake in the face of limited zinc availability. Mouse SLC39A4 is a 660 amino acid (aa) six transmembrane (TM) glycoprotein. It contains a long N-terminal extracellular region (aa 1-337) followed by six TM segments (aa 338-651) and a short C-terminal nine aa extracellular tail (aa 652-660). There is one splice variant that shows a 16 aa substitution for aa 1-63. SLC39A4 undergoes cell-specific proteolytic processing in response to low zinc concentrations. Cleavage occurs in the N-terminal extracellular region to generate a soluble 35 kDa polypeptide and a 37 kDa six TM integral membrane protein. This does not appear to be an inactivating mechanism. Over aa 1-337, mouse SLC39A4 shares 84% and 59% aa sequence identity with rat and human SLC39A4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.