Recombinant Human SIX6 His Protein

SDS-Page: Recombinant Human SIX6 His Protein [NBP2-22810] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human SIX6 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-246 of Human Homeobox protein SIX6
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMFQLPIL NFSPQQVAGV CETLEESGDV ERLGRFLWSL PVAPAACEAL NKNESVLRAR AIVAFHGGNY RELYHILENH KFTKESHAKL QALWLEAHYQ EAEKLRGRPL GPVDKYRVRK KFPLPRTIWD GEQKTHCFKE RTRNLLREWY LQDPYPNPSK KRELAQATGL TPTQVGNWFK NRRQRDRAAA AKNRLQQQVL SQGSGRALRA EGDGTPEVLG VATSPAASLS SKAATSAISI TSSDSECDI

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SIX6
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 30.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 40% glycerol, 5mM DTT, 2 mM EDTA
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human SIX6 His Protein

• homeobox protein SIX6
• Homeodomain protein OPTX2
• MCOPCT2
• Optic homeobox 2
• OPTX2
• sine oculis homeobox (Drosophila) homolog 6
• sine oculis homeobox homolog 6 (Drosophila)
• Sine oculis homeobox homolog 6
• SIX homeobox 6
• Six9

Background

SIX6, also as known as OPTX2 and SIX9, belongs to the SIX/Sine oculis homeobox family contains 1 homeobox DNA-binding domain. The SIX homeobox protein is crucial in embryonic development by providing necessary instructions for the formation of the forebrain and eye development. This protein expressed in the developing and adult retina, hypothalamic and the pituitary regions. Defects in SIX6 are the cause of microphthalmia isolated with cataract type 2. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Recombinant human SIX6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SIX6