SIX5 Antibody

Product Discontinued

SIX5 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 205-256 of Human Six5.
• Specificity: Six5 (L237) detects endogenous levels of Six5 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Six5 (L237) pAb in extracts from K562 cells.
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SIX5 Antibody

• BOR2
• DM locus-associated homeodomain protein
• DMAHPsine oculis homeobox homolog 5 (Drosophila)
• dystrophia myotonica-associated homeodomain protein
• homeobox protein SIX5
• sine oculis homeobox (Drosophila) homolog 5
• Sine oculis homeobox homolog 5
• SIX homeobox 5

Background

Six5 (homeobox protein SIX5), also known as SIX5, BOR2 or DMAHP (DM locus-associated homeodomain protein), is a transcription factor that is expressed in various structures of the adult eye. Localized to the cytoplasm in early development and to the nucleus in the later stages of development,Six5 is involved in regulation of organogenesis and in maintenance of retinal formation. Six5 is able to bind the 5'-TCA[AG][AG]TTNC-3' DNA sequence found in the myogenin and IGFBP5 promoters and, through this binding, can control transcription of the associated mRNA. Six5 is regulated via association with DACH1 (dachshund homolog 1) and is co-activated by the EYA (eyes absent) proteins. Defects in the gene encoding Six5 are the cause of branchiooto- renal syndrome type 2 (BOR2), an autosomal disorder characterized by hearing loss, a deep overbite and myopia. Two isoforms exist due to alternative splicing events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIX5
• Entrez:
  • 147912(Human)
  • 20475(Mouse)