SIRT7 Antibody

Western Blot: SIRT7 Antibody [NB110-81744] - WB detection of SIRT7 in tissue lysates of human liver using NB110-81744 antibody.

Immunocytochemistry/Immunofluorescence: SIRT7 Antibody [NB110-81744] - SIRT7 antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western Blot: SIRT7 Antibody [NB110-81744] - SIRT7 Immunoprecipitated from human liver lysates with NB110-81762. A). NB110-81746 B). NB110-81744

Product Discontinued

SIRT7 Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of human SIRT7 (within residues 100-200). [Swiss-Prot# Q9NRC8]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIRT7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:20 - 1:100
  • Western Blot 2 ug/ml
• Application Notes: This SIRT7 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~45 kDa. In ICC/IF nuclear staining was observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  SIRT7 Overexpression Lysate

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (88%), Drosophila (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SIRT7 Antibody

• EC 3.5.1.-
• MGC126840
• MGC126842
• silent mating type information regulation 2, S.cerevisiae, homolog 7
• SIR2L7
• SIR2L7NAD-dependent deacetylase sirtuin-7
• SIR2-like protein 7
• sir2-related protein type 7
• sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 7
• Sirtuin 7
• sirtuin type 7

Background

Sirtuins (SIRT) are highly conserved NAD-dependent deacetylases that deacetylate histone/non-histone proteins which regulates numerous cellular processes including aging, DNA repair, cell cycle, metabolism, and survival under stress conditions. NAD-dependent protein deacetylase sirtuin-7 (SIRT7 or SIR2L7) is one of the SIRTs whose function is least understood and it may be found at peri-nuclear region of cytoplasm; associated with chromatin, rDNA promoter, transcribed regionas or nucleolar organizer regions during mitosis. SIRT7's lacks the conserved amino acids within catalytic domain associated with deacetylase actions, however, with its weak deacetylase activity, it can deacetylate tumor suppressor p53 leading to enhanced cell survival. SIRT7 complexes with ELK4 to deacetylate H3K18 at the promoters of NME1, COPS2 genes and deacetylate histone H3K18 acetylation at promoters of RPS7, RPS14, RPS20, which function as tumor suppressor genes in hematopoietic malignancy. SIRT7 connects chromatin remodeling complexes with the RNA Pol I machinery required for rDNA driving ribosome biogenesis in dividing cells. It is overexpressed in human thyroid carcinoma cell lines and tissues, but not in adenomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRT7
• Entrez:
  • 51547(Human)
• Uniprot:
  • Q9NRC8(Human)