SIRT7 Antibody

Images

 
Western Blot: SIRT7 Antibody [NB110-81744] - WB detection of SIRT7 in tissue lysates of human liver using NB110-81744 antibody.
Immunocytochemistry/ Immunofluorescence: SIRT7 Antibody [NB110-81744] - SIRT7 antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Western Blot: SIRT7 Antibody [NB110-81744] - SIRT7 Immunoprecipitated from human liver lysates with NB110-81762. A). NB110-81746 B). NB110-81744

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

SIRT7 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human SIRT7 (within residues 100-200). [Swiss-Prot# Q9NRC8]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIRT7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:100
Application Notes
This SIRT7 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~45 kDa. In ICC/IF nuclear staining was observed.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Liver Lysate (NB820-59232)
SIRT7 Lysate (NBL1-15980)

Reactivity Notes

Human and mouse. Immunogen sequence has 88% homology to bovine and porcine and 82% homology to Drosophila.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SIRT7 Antibody

  • EC 3.5.1.-
  • MGC126840
  • MGC126842
  • silent mating type information regulation 2, S.cerevisiae, homolog 7
  • SIR2L7NAD-dependent deacetylase sirtuin-7
  • SIR2-like protein 7
  • sir2-related protein type 7
  • sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 7
  • sirtuin 7
  • sirtuin type 7

Background

Sirtuins (SIRT) are highly conserved NAD-dependent deacetylases that deacetylate histone/non-histone proteins which regulates numerous cellular processes including aging, DNA repair, cell cycle, metabolism, and survival under stress conditions. NAD-dependent protein deacetylase sirtuin-7 (SIRT7 or SIR2L7) is one of the SIRTs whose function is least understood and it may be found at peri-nuclear region of cytoplasm; associated with chromatin, rDNA promoter, transcribed regionas or nucleolar organizer regions during mitosis. SIRT7's lacks the conserved amino acids within catalytic domain associated with deacetylase actions, however, with its weak deacetylase activity, it can deacetylate tumor suppressor p53 leading to enhanced cell survival. SIRT7 complexes with ELK4 to deacetylate H3K18 at the promoters of NME1, COPS2 genes and deacetylate histone H3K18 acetylation at promoters of RPS7, RPS14, RPS20, which function as tumor suppressor genes in hematopoietic malignancy. SIRT7 connects chromatin remodeling complexes with the RNA Pol I machinery required for rDNA driving ribosome biogenesis in dividing cells. It is overexpressed in human thyroid carcinoma cell lines and tissues, but not in adenomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for SIRT7 Antibody (NB110-81744): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SIRT7 Antibody (NB110-81744) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NB110-81744V
FITC Labeled NB110-81744F

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Bioinformatics

Gene Symbol SIRT7
Entrez
Uniprot