Recombinant Human SIRT6 His Protein

SDS-Page: SIRT6 Protein [NBC1-18433] - SIRT6, 41 kDa (375 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human SIRT6 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-355 of Human SIRT6
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSVNYAAGLS PYADKGKCGL PEIFDPPEEL ERKVWELARL VWQSSSVVFH TGAGISTASG IPDFRGPHGV WTMEERGLAP KFDTTFESAR PTQTHMALVQ LERVGLLRFL VSQNVDGLHV RSGFPRDKLA ELHGNMFVEE CAKCKTQYVR DTVVGTMGLK ATGRLCTVAK ARGLRACRGE LRDTILDWED SLPDRDLALA DEASRNADLS ITLGTSLQIR PSGNLPLATK RRGGRLVIVN LQPTKHDRHA DLRIHGYVDE VMTRLMKHLG LEIPAWDGPR VLERALPPLP RPPTPKLEPK EESPTRINGS IPAGPKQEPC AQHNGSEPAS PKRERPTSPA PHRPPKRVKA KAVPS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SIRT6
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5), 20% glycerol, 1 mM DTT, 0.1 M NaCl, 0.1 mM PMSF
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human SIRT6 His Protein

• EC 3.5.1.-
• NAD-dependent deacetylase sirtuin-6
• SIR2L6sir2-related protein type 6
• SIR2-like protein 6
• sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
• sirtuin 6
• sirtuin type 6

Background

The SIRT6 is included in class IV of the sirtuin family, homologs to the yeast Sir2 protein. The functions of human sirtuins have not yet been determined; however, in yeast sirtuins are known to regulate epigenetic gene silencing. Also SIRT6, a chromatin-associated protein, appears to be involved in DNA repair. Recombinant SIRT6, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SIRT6