SIRT6 Antibody

Images

 
Western Blot: SIRT6 Antibody [NB100-2522] - WB analysis of SIRT6 in NIH/3T3 cell lysate.
Immunohistochemistry: SIRT6 Antibody [NB100-2522] - SIRT6 antibody was tested in mouse testis using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SIRT6 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region of the mouse SIRT6 protein (within residues 250-334). [Swiss-Prot P59941]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIRT6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:300
  • Immunoprecipitation
Application Notes
This SIRT6 antibody is useful for Western blot and Immunohistochemistry paraffin embedded sections. In Western blot a band is seen at ~37 kDa representing SIRT6, and a non-specific higher MW band can be seen at ~61 kDa. This antibody does not appear to detect the human protein in Western blot. In IHC-P, staining was observed in the nucleus of mouse testis. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23899523) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25366464) Use in Immunoprecipitation reported in scientific literature (PMID 25288139) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
MEF Lysate (NB800-PC10)
Control Peptide
SIRT6 Peptide (NB100-2522PEP)
Publications
Read Publications using
NB100-2522 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25288139) Immunogen sequence has 78% homology to human. Human reactivity reported in scientific literature (PMID: 25660418)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SIRT6 Antibody

  • EC 3.5.1.-
  • NAD-dependent deacetylase sirtuin-6
  • SIR2L6sir2-related protein type 6
  • SIR2-like protein 6
  • sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
  • sirtuin 6
  • sirtuin type 6

Background

SIRT6 (SIR2-like protein 6) is a member of the yeast silent information regulator 2 (SIR2) family and possesses both strong ADP-ribosyltransferase activity as well as protein deacetylase activity which is dependent on NAD(+). SIRT6 exerts deacetylase activity towards histone H3K9Ac and H3K56Ac, and alters histone H3 acetylation in telomeric chromatin during cell cycle's S-phase. It deacetylates histone H3K9Ac at NFkB target promoters and downregulates a subset of NFkB target genes. Upon DNA damage, SIRT6 promotes DNA end resection via deacetylation of RBBP8. SIRT6 also serves as a corepressor of the transcription factor HIF1 alpha for regulating the expression of multiple glycolytic genes to control glucose homeostasis, and is required for normal IGF1 serum levels. SIRT6 play critical roles in maintaining telomere integrity, fine-tuning aging-associated gene expression programs, preventing genomic instability, and maintaining metabolic homeostasis, thus impacting on several pathways relevant for cancer, metabolism, aging, cellular senescence and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF6347
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Publications for SIRT6 Antibody (NB100-2522)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ICC/IF
(3)
IHC
(2)
IHC-Fr
(1)
IHC-P
(2)
IP
(1)
WB
(7)
All Applications
Filter By Species
Human
(3)
Mouse
(5)
Rat
(2)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-2522 Applications Species
Tao NN, Ren JH, Tang H et al. Deacetylation of Ku70 by SIRT6 attenuates Bax-mediated apoptosis in hepatocellular carcinoma. Biochem. Biophys. Res. Commun. 2017 Feb 23 [PMID: 28238784] (WB) WB
Ran LK, Chen Y, Zhang ZZ et al. SIRT6 Overexpression Potentiates Apoptosis Evasion in Hepatocellular Carcinoma via BCL2-Associated X Protein-Dependent Apoptotic Pathway. Clin Cancer Res 2016 Jul 1 [PMID: 26861461]
Fukuda T, Wada-Hiraike O, Oda K et al. Putative tumor suppression function of SIRT6 in endometrial cancer FEBS Lett. 2015 Aug 04 [PMID: 26183563] (WB, Human)

Details:
SIRT6 antibody was used for Western Blot on SIRT6 knockdown in the AN3CA and KLE cells (Fig. 3B).
WB Human
Oikawa S, Mano A, Takahashi R, Kakinuma Y. Remote ischemic preconditioning with a specialized protocol activates the non-neuronal cardiac cholinergic system and increases ATP content in the heart Int. Immunopharmacol. 2015 Jun 11 [PMID: 26072685] (WB, Mouse) WB Mouse
Orellana ME, Quezada C, Maloney SC et al. Expression of SIRT2 and SIRT6 in Retinoblastoma Ophthalmic Res. 2015 Feb 09 [PMID: 25660418] (IHC-P, Human) IHC-P Human
Cardinale A, De Stefano Mc, Mollinari C et al. Biochemical Characterization of Sirtuin 6 in the Brain and Its Involvement in Oxidative Stress Response Neurochem. Res. 2014 Nov 01 [PMID: 25366464] (IHC-Fr, ICC/IF, WB, Mouse)

Details:
SIRT6 antibody used for IHC-Fr on 30uM coronal sectiions of cortical and hippocampal regions of mice (Fig 1). Antibody also used for ICC-IF on primary cortical and hippocampal neurons from E18 mice (Fig 3a). Antibody also used for WB of SIRT6 in cortical and hippocampal regions of mice (Fig 2), on cultured primary cortical / hippocampal neurons from E18 mice (Fig 3B) and on whole protein extracts derived from uninfected, rAAV-HA and rAAV-HA-SIRT6 infected cortical neuron (Fig 4B)
IHC-Fr, ICC/IF, WB Mouse
Tran L, Schulkin J, Ligon Co et al. Epigenetic modulation of chronic anxiety and pain by histone deacetylation. Mol. Psychiatry. 2014 Oct 07 [PMID: 25288139] (ICC/IF, IP, Rat) ICC/IF, IP Rat
Thirumurthi Umadevi, Shen Jia, Xia Weiya et al. MDM2-mediated degradation of SIRT6 phosphorylated by AKT1 promotes tumorigenesis and trastuzumab resistance in breast cancer. Sci Signal. 2014 Jul 29 [PMID: 25074979] (IHC) IHC
Lee OH, Kim J, Kim JM et al. Decreased expression of sirtuin 6 is associated with release of high mobility group box-1 after cerebral ischemia. Biochem Biophys Res Commun 2013 Jul 27 [PMID: 23899523] (ICC/IF, Rat) ICC/IF Rat
Sundaresan NR, Vasudevan P, Zhong L et al. The sirtuin SIRT6 blocks IGF-Akt signaling and development of cardiac hypertrophy by targeting c-Jun Nat Med 2012 Oct 21 [PMID: 23086477] (WB, Mouse) WB Mouse
Show All 12 Publications.

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Product General Protocols

View specific protocols for SIRT6 Antibody (NB100-2522): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SIRT6 Antibody (NB100-2522) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2522AF405
Alexa Fluor 488 Labeled NB100-2522AF488
Alexa Fluor 647 Labeled NB100-2522AF647
Alexa Fluor 700 Labeled NB100-2522AF700
Biotin Labeled NB100-2522B
DyLight 405 Labeled NB100-2522V
DyLight 488 Labeled NB100-2522G
DyLight 550 Labeled NB100-2522R
DyLight 650 Labeled NB100-2522C
FITC Labeled NB100-2522F
HRP Labeled NB100-2522H

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Bioinformatics

Gene Symbol SIRT6