This SIRT6 antibody is useful for Western blot and Immunohistochemistry paraffin embedded sections. In Western blot a band is seen at ~37 kDa representing SIRT6, and a non-specific higher MW band can be seen at ~61 kDa. This antibody does not appear to detect the human protein in Western blot. In IHC-P, staining was observed in the nucleus of mouse testis. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23899523) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25366464) Use in Immunoprecipitation reported in scientific literature (PMID 25288139) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rat reactivity reported in scientific literature (PMID: 25288139) Immunogen sequence has 78% homology to human. Human reactivity reported in scientific literature (PMID: 25660418)
Alternate Names for SIRT6 Antibody
NAD-dependent deacetylase sirtuin-6
SIR2L6sir2-related protein type 6
SIR2-like protein 6
sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
sirtuin type 6
SIRT6 (SIR2-like protein 6) is a member of the yeast silent information regulator 2 (SIR2) family and possesses both strong ADP-ribosyltransferase activity as well as protein deacetylase activity which is dependent on NAD(+). SIRT6 exerts deacetylase activity towards histone H3K9Ac and H3K56Ac, and alters histone H3 acetylation in telomeric chromatin during cell cycle's S-phase. It deacetylates histone H3K9Ac at NFkB target promoters and downregulates a subset of NFkB target genes. Upon DNA damage, SIRT6 promotes DNA end resection via deacetylation of RBBP8. SIRT6 also serves as a corepressor of the transcription factor HIF1 alpha for regulating the expression of multiple glycolytic genes to control glucose homeostasis, and is required for normal IGF1 serum levels. SIRT6 play critical roles in maintaining telomere integrity, fine-tuning aging-associated gene expression programs, preventing genomic instability, and maintaining metabolic homeostasis, thus impacting on several pathways relevant for cancer, metabolism, aging, cellular senescence and apoptosis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.