SIRP gamma/CD172g Antibody [Alexa Fluor™ Plus 405]

• Reactivity: Hu
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor Plus 405

SIRP gamma/CD172g Antibody [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects human SIRP gamma /CD172g in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) SIRP alpha , rhSIRP beta 1, and recombinant mouse SIRP alpha is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIRP gamma/CD172g Antibody [Alexa Fluor™ Plus 405]

• bA77C3.1
• CD172g antigen
• CD172g
• FLJ42230
• signal-regulatory protein beta 2
• Signal-regulatory protein beta-2
• signal-regulatory protein gamma
• SIRP beta 2
• SIRP gamma
• SIRP-B2
• SIRPB2CD172 antigen-like family member B
• SIRP-beta-2
• SIRPG
• SIRPgamma
• SIRP-gamma

Background

Signal regulatory protein gamma (SIRP gamma , designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane glycoprotein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily (1 ‑ 5). SIRP members are “paired receptors” with homology in the extracellular domain but variability in the C‑terminus and signaling function (1, 2). The 387 amino acid (aa) SIRP gamma sequence contains a 28 aa potential signal sequence, a 332 aa extracellular domain (ECD) with four potential N‑glycosylation sites, a 23 aa transmembrane domain and a 4 aa cytoplasmic sequence. SIRP gamma contains one V-type Ig‑like domain that contains a J‑like sequence and two C1-type Ig‑like domains within its ECD (1, 2). Isoforms that lack one (isoform 2, 276 aa) or two (isoform 3, 170 aa) membrane-proximal C‑type Ig-like domains have been described (5). Within the ECD, human SIRP gamma isoform 1 shares 78% aa identity with human SIRP beta 1, and appears to have structurally similar orthologs only in rhesus monkey and chimpanzee (100% and 91% aa identity, respectively) (2). SIRP gamma is the only SIRP known to be expressed on T cells, CD56^bright NK cells and activated NK cells; it is not expressed on myeloid cells (5, 6). It shows adhesion to CD47, but at lower affinity than SIRP alpha (6). Expression of SIRP gamma on T cells suggests a role as an accessory protein interacting with CD47‑expressing antigen presenting cells (5, 6). Unlike SIRP alpha that has cytoplasmic ITIM domains, and SIRP beta 1 that interacts with DAP-12, SIRP gamma does not contain any obvious signaling mechanism (1, 2, 6). However, SIRP gamma -mediated adhesion appears to promote antigen-specific T cell proliferation and costimulate T cell activation (5).

1. Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.
2. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
3. van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
4. Ichigotani, Y. et al. (2000) J. Hum. Genet. 45:378.
5. Piccio, L. et al. (2005) Blood 105:2421.
6. Brooke, G. et al. (2004) J. Immunol. 173:2562.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.