SIRP gamma/CD172g Antibody [Unconjugated]

Western blot shows lysates of NK-92 human natural killer lymphoma cell line, Jurkat human acute T cell leukemia cell line, and human thymus tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SIRP gamma/CD172g Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4486) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). Specific bands were detected for SIRP gamma/CD172g at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human peripheral blood monocytes were stained with Sheep Anti-Human SIRP gamma/CD172g Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4486) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (F0126) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SIRP gamma/CD172g Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human SIRP gamma /CD172g
  Val64-Ser364, (Val263Ala) and (Ser286Leu)
  Accession # Q9P1W8
• Specificity: Detects human SIRP gamma /CD172g in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) SIRP alpha , rhSIRP beta 1, and recombinant mouse SIRP alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SIRPG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIRP gamma/CD172g Antibody [Unconjugated]

• bA77C3.1
• CD172g antigen
• CD172g
• FLJ42230
• signal-regulatory protein beta 2
• Signal-regulatory protein beta-2
• signal-regulatory protein gamma
• SIRP beta 2
• SIRP gamma
• SIRP-B2
• SIRPB2CD172 antigen-like family member B
• SIRP-beta-2
• SIRPG
• SIRPgamma
• SIRP-gamma

Background

Signal regulatory protein gamma (SIRP gamma , designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane glycoprotein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily (1 - 5). SIRP members are “paired receptors” with homology in the extracellular domain but variability in the C-terminus and signaling function (1, 2). The 387 amino acid (aa) SIRP gamma sequence contains a 28 aa potential signal sequence, a 332 aa extracellular domain (ECD) with four potential N-glycosylation sites, a 23 aa transmembrane domain and a 4 aa cytoplasmic sequence. SIRP gamma contains one V-type Ig-like domain that contains a J-like sequence and two C1-type Ig-like domains within its ECD (1, 2). Isoforms that lack one (isoform 2, 276 aa) or two (isoform 3, 170 aa) membrane-proximal C-type Ig-like domains have been described (5). Within the ECD, human SIRP gamma isoform 1 shares 78% aa identity with human SIRP beta 1, and appears to have structurally similar orthologs only in rhesus monkey and chimpanzee (100% and 91% aa identity, respectively) (2). SIRP gamma is the only SIRP known to be expressed on T cells, CD56 bright NK cells and activated NK cells; it is not expressed on myeloid cells (5, 6). It shows adhesion to CD47, but at lower affinity than SIRP alpha (6). Expression of SIRP gamma on T cells suggests a role as an accessory protein interacting with CD47-expressing antigen presenting cells (5, 6). Unlike SIRP alpha that has cytoplasmic ITIM domains, and SIRP beta 1 that interacts with DAP-12, SIRP gamma does not contain any obvious signaling mechanism (1, 2, 6). However, SIRP gamma -mediated adhesion appears to promote antigen-specific T cell proliferation and costimulate T cell activation (5).

1. Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.
2. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
3. van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
4. Ichigotani, Y. et al. (2000) J. Hum. Genet. 45:378.
5. Piccio, L. et al. (2005) Blood 105:2421.
6. Brooke, G. et al. (2004) J. Immunol. 173:2562.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRPG
• Uniprot:
  • Q9P1W8()