![Western Blot: Sir2.1 Antibody (SN70-04) [NBP2-67707] - Western blot analysis of Sir2.1 on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/Sir2-1-Antibody-SN70-04-Western-Blot-NBP2-67707-img0004.jpg "Western Blot: Sir2.1 Antibody (SN70-04) [NBP2-67707] - Western blot analysis of Sir2.1 on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab")
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Sir2.1 aa 335-389 / 389. (SwissProt: Q8IXJ6 Human; SwissProt: Q5RJQ4 Rat) |
| Localization | Nucleus, Cytoplasm, Midbody, Chromosome, Cell projection, Myelin membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SIRT2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Sir2.1 Antibody (NBP2-67707)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SIRT2 |
![Immunocytochemistry/Immunofluorescence: Sir2.1 Antibody (SN70-04) [NBP2-67707] - Staining SIRT2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Sir2-1-Antibody-SN70-04-Immunocytochemistry-Immunofluorescence-NBP2-67707-img0003.jpg "Immunocytochemistry/Immunofluorescence: Sir2.1 Antibody (SN70-04) [NBP2-67707] - Staining SIRT2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Flow Cytometry: Sir2.1 Antibody (SN70-04) [NBP2-67707] - Analysis of Hela cells with SIRT2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Sir2-1-Antibody-SN70-04-Flow-Cytometry-NBP2-67707-img0001.jpg "Flow Cytometry: Sir2.1 Antibody (SN70-04) [NBP2-67707] - Analysis of Hela cells with SIRT2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody")
![Immunocytochemistry/Immunofluorescence: Sir2.1 Antibody (SN70-04) [NBP2-67707] - Staining SIRT2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Sir2-1-Antibody-SN70-04-Immunocytochemistry-Immunofluorescence-NBP2-67707-img0002.jpg "Immunocytochemistry/Immunofluorescence: Sir2.1 Antibody (SN70-04) [NBP2-67707] - Staining SIRT2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
