SIPA1 Antibodies
We offer SIPA1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot. Each SIPA1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SIPA1 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the SIPA1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SIPA1 polyclonal antibodies.
Alternate Names for SIPA1 Antibodies
anti-SIPA1 antibody, anti-GTPase-activating protein Spa-1 antibody, anti-MGC102688 antibody, anti-MGC17037 antibody, anti-p130 SPA-1 antibody, anti-signal-induced proliferation-associated 1 antibody, anti-signal-induced proliferation-associated protein 1 antibody, anti-sipa-1 antibody, anti-SPA1signal-induced proliferation-associated gene 1 antibody
13 Results for "sipa1" in Primary Antibodies
![Western Blot: SIPA1 Antibody [NBP2-20369] - Western Blot analysis of SIPA1 expression in transfected 293T cell line by SIPA1 polyclonal antibody. A: Non-transfected lysate. B: SIPA1 transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/SIPA1-Antibody-Western-Blot-NBP2-20369-img0001.jpg "Western Blot: SIPA1 Antibody [NBP2-20369] - Western Blot analysis of SIPA1 expression in transfected 293T cell line by SIPA1 polyclonal antibody. A: Non-transfected lysate. B: SIPA1 transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: SIPA1 Antibody [NBP2-20369] - Analysis of methanol-fixed mouse ESC, using SIPA1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SIPA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20369-img0002.jpg)
![Immunohistochemistry-Paraffin: SIPA1 Antibody [NBP2-20369] - Paraffin-embedded HBL435 xenograft. SIPA1 antibody [C3], C-term dilution: 1:500.](http://images.novusbio.com/fullsize/SIPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-20369-img0004.jpg)
![Immunohistochemistry-Paraffin: SIPA1 Antibody [NBP2-20369] - Paraffin-embedded Cal27 xenograft. SIPA1 antibody [C3], C-term dilution: 1:500.](http://images.novusbio.com/fullsize/SIPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-20369-img0003.jpg)
![Western Blot: SIPA1 Antibody [NBP1-77947] - Detection of over-expressed Sipa1 in lysates from mouse 3T3 cells transfected with Sipa1 (lane 1). Endogenous Sipa1 is detected in lane 2, which contains lysate from 3T3 cells mock-transfected with LacZGLB, although at a significantly reduced level compared to transfected cells. Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane. The identity of the higher and lower molecular weight bands is unknown. The band at ~130 kDa, indicated by the arrowhead, corresponds to recombinant Sipa1. Primary antibody was used at 1:1250.](http://images.novusbio.com/fullsize/antibody/SIPA1-Antibody-Western-Blot-NBP1-77947-img0002.jpg "Western Blot: SIPA1 Antibody [NBP1-77947] - Detection of over-expressed Sipa1 in lysates from mouse 3T3 cells transfected with Sipa1 (lane 1). Endogenous Sipa1 is detected in lane 2, which contains lysate from 3T3 cells mock-transfected with LacZGLB, although at a significantly reduced level compared to transfected cells. Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane. The identity of the higher and lower molecular weight bands is unknown. The band at ~130 kDa, indicated by the arrowhead, corresponds to recombinant Sipa1. Primary antibody was used at 1:1250.")
![Immunohistochemistry: SIPA1 Antibody [NBP1-77947] - Tissue: small intestine. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-Sipa1 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sipa-1 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/antibody/SIPA1-Antibody-Immunohistochemistry-NBP1-77947-img0005.jpg)
![Immunohistochemistry: SIPA1 Antibody [NBP1-77947] - Affinity purified anti-Sipa1 antibody was used at 1.25 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/nbp1-77947_rabbit-polyclonal-sipa1-antibody-235202318143119.jpg)
![Immunohistochemistry-Paraffin: SIPA1 Antibody [NBP2-32620] - Staining in human tonsil and pancreas tissues using anti-SIPA1 antibody. Corresponding SIPA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SIPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-32620-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SIPA1 Antibody [NBP2-32620] - Staining in human tonsil and pancreas tissues using anti-SIPA1 antibody. Corresponding SIPA1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SIPA1 Antibody [NBP2-32620] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli & plasma membrane.](http://images.novusbio.com/fullsize/SIPA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32620-img0006.jpg)
![Immunohistochemistry-Paraffin: SIPA1 Antibody [NBP2-32620] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SIPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-32620-img0007.jpg)
![Immunohistochemistry-Paraffin: SIPA1 Antibody [NBP2-32620] - Staining of human tonsil shows strong cytoplasmic positivity in non germinal center cells.](http://images.novusbio.com/fullsize/SIPA1-Antibody-Immunohistochemistry-NBP2-32620-img0001.jpg)
![Western Blot: SIPA1 Antibody [NBP2-98740] - Anti-SIPA1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 112 kDa. Observed band size: 115 kDa](http://images.novusbio.com/fullsize/SIPA1-Antibody-Western-Blot-NBP2-98740-img0002.jpg "Western Blot: SIPA1 Antibody [NBP2-98740] - Anti-SIPA1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 112 kDa. Observed band size: 115 kDa")
![Immunocytochemistry/Immunofluorescence: SIPA1 Antibody [NBP2-98740] - Immunofluorescence staining of SIPA1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human SIPA1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/SIPA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98740-img0001.jpg)
![Immunohistochemistry-Paraffin: SIPA1 Antibody [NBP2-98740] - Immunochemical staining of human SIPA1 in human kidney with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SIPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-98740-img0003.jpg)
![Western Blot: SIPA1 Antibody [NBP2-81850] - Analysis of SIPA1 in human brain tissue lysate with SIPA1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/SIPA1-Antibody-Western-Blot-NBP2-81850-img0001.jpg "Western Blot: SIPA1 Antibody [NBP2-81850] - Analysis of SIPA1 in human brain tissue lysate with SIPA1 antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: SIPA1 Antibody - BSA Free [NBP2-81850] - Immunofluorescence of SIPA1 in human brain cells with SIPA1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81850_rabbit-polyclonal-sipa1-antibody-2711202420324856.jpg)
![Western Blot: SIPA1 Antibody [NB100-1390] - Analysis of SIPA1 in Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35ug total protein per lane) at 1ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/SIPA1-Antibody-Western-Blot-NB100-1390-img0005.jpg "Western Blot: SIPA1 Antibody [NB100-1390] - Analysis of SIPA1 in Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35ug total protein per lane) at 1ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Western Blot: SIPA1 Antibody [NBP1-18917] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. SIPA1 was also immunoprecipitated by another rabbit anti-SIPA1 antibody.](http://images.novusbio.com/fullsize/SIPA1-Antibody-Western-Blot-NBP1-18917-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SIPA1 Antibody [NBP1-18917] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. SIPA1 was also immunoprecipitated by another rabbit anti-SIPA1 antibody.")
![Western Blot: SIPA1 Antibody [NBP3-35235] - Western blot analysis of various lysates using SIPA1 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35235_rabbit-sipa1-pab-912202414201317.jpg "Western Blot: SIPA1 Antibody [NBP3-35235] - Western blot analysis of various lysates using SIPA1 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")