SIPA1 Antibody - BSA Free

Western Blot: SIPA1 Antibody [NBP1-18917] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. SIPA1 was also immunoprecipitated by another rabbit anti-SIPA1 antibody.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SIPA1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SIPA1 Antibody - BSA Free (NBP1-18917) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 992 to 1042 of human signal-induced proliferation-associated protein 1 using the numbering given in entry NP_006738.3 (GeneID 6494).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIPA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SIPA1 Antibody - BSA Free

• GTPase-activating protein Spa-1
• MGC102688
• MGC17037
• p130 SPA-1
• signal-induced proliferation-associated 1
• signal-induced proliferation-associated protein 1
• sipa-1
• SPA1signal-induced proliferation-associated gene 1

Background

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIPA1
• Entrez:
  • 6494(Human)
  • 20469(Mouse)
• Uniprot:
  • Q96FS4(Human)