SIPA1 Antibody

Western Blot: SIPA1 Antibody [NB100-1390] - Analysis of SIPA1 in Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35ug total protein per lane) at 1ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SIPA1 Antibody Summary

• Immunogen: Peptide with sequence C-ASKQLGSPTADLA corresponding to C-Terminus according to NP_694985.28, NP_006738.3.
• Localization: Perinuclear
• Specificity: Both variants represent identical protein (NP_694985.28 and NP_006738.3).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SIPA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 150 kDa band observed in human lysates of EBV-immortilized lymphoblastoid is reported in scientific literature (PMID: 9346962). An additional band of 55-60 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than WB.
• Control:

  Jurkat Whole Cell Lysate
  Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SIPA1 Antibody

• GTPase-activating protein Spa-1
• MGC102688
• MGC17037
• p130 SPA-1
• signal-induced proliferation-associated 1
• signal-induced proliferation-associated protein 1
• sipa-1
• SPA1signal-induced proliferation-associated gene 1

Background

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIPA1 Antibody (NB100-1390)(1)

Publication using NB100-1390

• Hattori M, Tsukamoto N, Nur-e-Kamal MS et al. Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression. Mol Cell Biol 1995-01-01 [PMID: 7799964]

Bioinformatics

• Gene Symbol: SIPA1
• Entrez:
  • 6494(Human)
• Uniprot:
  • NP_006738(Human)