SIM2 Antibody

Immunocytochemistry/Immunofluorescence: SIM2 Antibody [NBP1-88619] - Staining of human cell line U-251 MG shows localization to nucleoplasm and nuclear bodies. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SIM2 Antibody [NBP1-88619] - Staining of human Rectum shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: SIM2 Antibody [NBP1-88619] - Staining of human Kidney shows moderate nuclear positivity in cells in glomeruli and tubules.

Immunohistochemistry-Paraffin: SIM2 Antibody [NBP1-88619] - Staining of human Placenta shows strong nuclear positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: SIM2 Antibody [NBP1-88619] - Staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts.

Product Discontinued

SIM2 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: CIVSVNYVLTEIEYKELQLSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SIM2 Antibody

• human transcription factor SIM2, homolog of the Drosophila single-minded geneSIM110BHLHE15
• short isoform
• single-minded (Drosophila) homolog 2
• single-minded homolog 2 (Drosophila)
• transcription factor SIM2

Background

SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. The Drosophila sim gene encodes a transcription factor that is a master regulator of fruit fly neurogenesis. SIM2 maps within the so-called Down syndrome chromosomal region. Based on the mapping position, its potential function as transcriptional repressor and similarity to Drosophila sim, it is proposed that SIM2 may contribute to some specific Down syndrome phenotypes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIM2