Siglec-E Antibody (750620)

Mouse Splenocytes were stained with Anti-Mouse Integrin alpha X/CD11c Phycoerythrin-conjugated Antibody and either (A) Rat IgG2B Isotype Control (Catalog # MAB0061) or (B) Rat Anti-Mouse Siglec-E Monoclonal Antibody (Catalog # MAB5806) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 750620
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Siglec-E Antibody (750620) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Siglec-E
  Gln19-Phe354
  Accession # Q91Y57
• Specificity: Detects mouse Siglec-E in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Siglec-3/CD33 or rmSiglec-F is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Siglece
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-E Antibody (750620)

• CD170;CD33L2;OBBP2;OB-BP2;Sialic acid-binding Ig-like lectin 5;SIGLEC5;SIGLEC-5
• SiglecE
• Siglec-E

Background

Mouse Siglec-E (Salic acid binding Ig-like lectin E), also known as Siglecl1, is a 52 kDa (predicted) member of the CD33-related subfamily of Siglecs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-E Antibody (MAB5806)(3)

Publications using MAB5806

• G Dharmadhik, K Stolz, M Hauke, NG Morgan, A Varki, E de Koning, S Kelm, K Maedler Siglec-7 restores ?-cell function and survival and reduces inflammation in pancreatic islets from patients with diabetes Sci Rep, 2017-04-05;7(0):45319. 2017-04-05 [PMID: 28378743] (Flow Cytometry, Human)
• Siglec-E Negatively Regulates the Activation of TLR4 by Controlling Its Endocytosis J Immunol, 2016-09-12;0(0):. 2016-09-12 [PMID: 27619995] (Flow Cytometry, Mouse)
• D.G. Walker, A.M. Whetzel, L.-F. Lue Expression of suppressor of cytokine signaling genes in human elderly and Alzheimer’s disease brains and human microglia Neuroscience 8/27/2015 [PMID: 25286386]

Bioinformatics

• Gene Symbol: Siglece
• Uniprot:
  • Q91Y57()