Siglec-E Antibodies

HAF109). A specific band was detected for Siglec-E at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF109). A specific band was detected for Siglec-E at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Siglec-E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5806) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Siglec-E at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

MAB0061) or (B) Rat Anti-Mouse Siglec-E Monoclonal Antibody (Catalog # MAB5806) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_lightbox"> MAB0061) or (B) Rat Anti-Mouse Siglec-E Monoclonal Antibody (Catalog # MAB5806) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." alt="Mouse Splenocytes were stained with Anti-Mouse Integrin aX/CD11c Phycoerythrin-conjugated Antibody and either (A) Rat IgG2B Isotype Control (Catalog # MAB0061) or (B) Rat Anti-Mouse Siglec-E Monoclonal Antibody (Catalog # MAB5806) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_thumb" />