Siglec-6/CD327 Antibody (767329) [Phycoerythrin]

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Siglec‑6/CD327 PE‑conjugated Monoclonal Antibody (Catalog # FAB2859P, filled histogram) or isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human Siglec-6/CD327 PE-conjugated Monoclonal Antibody (Catalog # FAB2859P) or (B) Mouse Phycoerythrin Isotype Control. View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 767329
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Siglec-6/CD327 Antibody (767329) [Phycoerythrin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Siglec‑6/CD327
  Gln27-Val331
  Accession # NP_942142
• Specificity: Detects human Siglec‑6/CD327 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIGLEC6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-6/CD327 Antibody (767329) [Phycoerythrin]

• CD327 antigen
• CD327
• CD33 antigen-like 1
• CD33L1
• CD33L1OBBP1CD327
• CD33L2
• CD33LCDW327
• CDw327
• OB-BP1
• Obesity-binding protein 1
• sialic acid binding Ig-like lectin 6
• sialic acid-binding Ig-like lectin 6
• Siglec6
• Siglec-6

Background

Siglecs (Sialic acid binding Ig-like Lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1-4). Eleven human Siglecs (Siglec-1 through 11) have been cloned and characterized. Within these eleven, there are at least two groups, one of which is termed the CD33-related group. CD33-related Siglecs include CD33/Siglec-3 and Siglec-5 through 11 (1, 3). To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid. This suggests that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. The cDNA of human Siglec-6 (also known as OB-BP1 and CD33L), encodes a putative 442 amino acid (aa) protein that contains a 15 aa signal peptide, a 321 aa extracellular region, a 21 aa transmembrane region (TM), and an 85 aa cytoplasmic tail (5, 6). The extracellular region contains one N-terminal V-type Ig-like domain followed by two Ig-like C2-type domains. The cytoplasmic domain has one immunoreceptor tyrosine-based inhibition motif (ITIM). At least three additional isoforms exist, all of which encode an additional 11 aa’s at the N-terminus, likely due to the utilization of an alternate start site. Two of the three isoforms also show splicing. One isoform shows a 16 aa in-frame deletion in the second C2-like domain, while the other shows a deletion of the TM and cytoplasmic region, thus potentially generating a soluble form (6-9). Siglec-6 is found on B cells and in placenta, and would seem to have a restricted specificity for the sialyl Tn antigen (6, 10).

1. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
2. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
3. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
4. Powell, L.D. and A. Varki (1995) J. Biol. Chem. 270:14243.
5. Takei, Y. et al. (1997) Cytogenet. Cell Genet. 78:295.
6. Patel, N. et al. (1999) J. Biol. Chem. 274:22729.
7. GenBank Accession # NP_942143.
8. GenBank Accession # NP_942142.
9. GenBank Accession # NP_001236.
10. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-6/CD327 Antibody (FAB2859P)(4)

Publications using FAB2859P

• Du, F;Rische, CH;Li, Y;Vincent, MP;Krier-Burris, RA;Qian, Y;Yuk, SA;Almunif, S;Bochner, BS;Qiao, B;Scott, EA; Controlled adsorption of multiple bioactive proteins enables targeted mast cell nanotherapy Nature nanotechnology 2024-01-16 [PMID: 38228804] (Flow Cytometry, Human)
• Sulczewski, FB;Maqueda-Alfaro, RA;Alcántara-Hernández, M;Perez, OA;Saravanan, S;Yun, TJ;Seong, D;Arroyo Hornero, R;Raquer-McKay, HM;Esteva, E;Lanzar, ZR;Leylek, RA;Adams, NM;Das, A;Rahman, AH;Gottfried-Blackmore, A;Reizis, B;Idoyaga, J; Transitional dendritic cells are distinct from conventional DC2 precursors and mediate proinflammatory antiviral responses Nature immunology 2023-07-06 [PMID: 37414907] (Flow Cytometry, Human)
• F Du, C Rische, Y Li, M Vincent, R Krier-Burr, Y Qian, S Yuk, S Almunif, B Bochner, B Qiao, E Scott Bioactive multi-protein adsorption enables targeted mast cell nanotherapy Research square, 2023-01-26;0(0):. 2023-01-26 [PMID: 36747749] (Flow Cytometry, Human)
• R Leylek, M Alcántara-, JM Granja, M Chavez, K Perez, OR Diaz, R Li, AT Satpathy, HY Chang, J Idoyaga Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity Cell Rep, 2020-09-22;32(12):108180. 2020-09-22 [PMID: 32966789] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: SIGLEC6
• Uniprot:
  • NP_942142()