Siglec-1/CD169 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Siglec-1/CD169 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Siglec‑1/CD169
  Thr20-Leu1639
  Accession # Q62230
• Specificity: Detects mouse Siglec‑1/CD169 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Siglec-1 is observed and less than 1% cross-reactivity with rhSiglec-2 and recombinant mouse Siglec-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SIGLEC1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-1/CD169 Antibody [Biotin]

• CD169
• FLJ00051
• sialic acid binding Ig-like lectin 1, sialoadhesin
• sialoadhesin
• Siglec1
• Siglec-1

Background

Siglecs are sialic acid specific I-type lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N-terminal Ig-like V‑set domain followed by varying numbers of Ig-like C2-set domains (1, 2). Mouse Siglec-1, also known as sialoadhesin and CD169, is a 175-185 kDa glycoprotein that consists of a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains, a 21 aa transmembrane segment, and a 35 aa cytoplasmic domain (3, 4). Within the ECD, mouse Siglec-1 shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates a soluble form of the ECD and a soluble isoform that is truncated following the first three Ig-like domains (3). Siglec-1 expression is restricted to lymph node and spleen macrophages and some tissue macrophages (4). The adhesive function of Siglec-1 is supported by the N-terminal Ig-like domain which shows a selectivity for alpha -2,3-linked sialic acid residues (4-6). Siglec-1 binds a number of sialylated molecules including the mannose receptor, MGL1, MUC1, PSGL-1, and different glycoforms of CD43 (7-10). Its binding capacity can be masked by endogenous sialylated molecules (11, 12). The sialylated and sulfated N-linked carbohydrates that modify Siglec-1 itself are required for ligand binding (7, 8). Siglec-1 is expressed on dendritic cells following rhinovirus exposure, and these DC promote T cell anergy (13). It is also induced on circulating monocytes during systemic sclerosis and HIV-1 infection (14-16). Siglec-1 can trap HIV-1 particles for trans infection of permissive cells (15).

1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
2. Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
3. Crocker, P.R. et al. (1994) EMBO J. 13:4490.
4. Hartnell, A. et al. (2001) Blood 97:288.
5. Nath, D. et al. (1995) J. Biol. Chem. 270:26184.
6. Crocker, P.R. et al. (1991) EMBO J. 10:1661.
7. Martinez-Pomares, L. et al. (1999) J. Biol. Chem. 274:35211.
8. Kumamoto, Y. et al. (2004) J. Biol. Chem. 279:49274.
9. Nath, D. et al. (1999) Immunology 98:213.
10. van den Berg, T.K. et al. (2001) J. Immunol. 166:3637.
11. Nakamura, K. et al. (2002) Glycobiology 12:209.
12. Barnes, Y.C. et al. (1999) Blood 93:1245.
13. Kirchberger, S. et al. (2005) J. Immunol. 175:1145.
14. York, M.R. et al. (2007) Arthritis Rheum. 56:1010.
15. Rempel, H. et al. (2008) PloS ONE 3:e1967.
16. van der Kuyl, A.C. et al. (2007) Plos ONE 2:e257.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-1/CD169 Antibody (BAF5610)(1)

Publication using BAF5610

• Caroline L. Sokol, Ryan B. Camire, Michael C. Jones, Andrew D. Luster The chemokine receptor CCR8 promotes the migration of dendritic cells into the lymph node parenchyma to initiate the allergic immune response. Immunity 2018-09-01 [PMID: 30170811]

Bioinformatics

• Gene Symbol: SIGLEC1
• Uniprot:
  • Q62230()