Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) - Azide and BSA Free

Flow Cytometry: Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) [NBP3-08990] - Murine bone marrow-derived macrophages (BMDMs) were stained with anti-Siglec-1/CD169 antibody NBP3-08990 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor(R) 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a Siglec-1/CD169-positive population is apparent.

• Reactivity: Mu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: 3D6.112 (recombinant version)
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Spleen cells from AO rats immunised with thioglycollate-elicted murine peritoneal macrophages (TPM) that had been cutured in mouse serum to induce sheep erythrocyte receptor (SER).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: SIGLEC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: This constant region of this antibody has been modified to bind to Protein A, allowing highly-specific purification of this antibody by Protein A-affinity column.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) - Azide and BSA Free

• CD169
• FLJ00051
• sialic acid binding Ig-like lectin 1, sialoadhesin
• sialoadhesin
• Siglec1
• Siglec-1

Background

The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIGLEC1