SHC1 Antibody

Western Blot: SHC1 Antibody [NBP3-23392] - Western blot analysis of SHC1 expression in A431 cell lysate (lanes 1, 2, 3, & 4). The blots were probed with rabbit polyclonal anti-SHC1 (NBP3-23392) at 1:1000 (lane 1) or 1:4000 (lane 2) and mouse monoclonal SHC1 (C-terminal region) at 1:1000 (lane 3) or 1:4000 (lane 4).

Immunocytochemistry/Immunofluorescence: SHC1 Antibody [NBP3-23392] - Immunocytochemical labeling of SHC1 in paraformaldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal (top) and rabbit polyclonal (bottom NBP3-23392) SHC1 antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

• Reactivity: Hu, Mu, Rt, Rb
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SHC1 Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: A recombinant protein that included amino acid residues within the C-terminal region of human SHC1. This sequence has high homology with similar regions in rat and mouse SHC1. [UniProt# P29353]
• Specificity: The antibody detects 46/52/66 kDa bands corresponding to the molecular weight of SHC1 variants on SDS-PAGE immunoblots of A431 cells. It also detects SHC1 in human Jurkat, HeLa, and SKN-SH cells, as well as rat brain tissue and rabbit spleen fibroblasts.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SHC1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:1000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for SHC1 Antibody

• FLJ26504
• p66
• p66SHC
• SH2 domain protein C1
• SHC (Src homology 2 domain containing) transforming protein 1
• SHC (Src homology 2 domain-containing) transforming protein 1
• SHC
• SHC1
• SHCA
• SHC-transforming protein 1
• SHC-transforming protein 3
• SHC-transforming protein A
• Src homology 2 domain-containing-transforming protein C1

Background

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc andisoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of theGRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoformp52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shcdoes not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular responseto oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and isindispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome crelease and apoptosis. The expression of isoform p66Shc has been correlated with life span

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SHC1