SHC1 Antibody - BSA Free

Western Blot: SHC1 Antibody [NBP2-20352] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SHC1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: SHC1 Antibody [NBP2-20352] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHC1 antibody [N3C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: SHC1 Antibody [NBP2-20352] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:3000.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SHC1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SHC1. The exact sequence is proprietary.
• Localization: Cytoplasm; Isoform p46Shc: Mitochondrion matrix
• Predicted Species: Rat (91%), Bovine (94%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SHC1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (81%), Chicken (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SHC1 Antibody - BSA Free

• FLJ26504
• p66
• p66SHC
• SH2 domain protein C1
• SHC (Src homology 2 domain containing) transforming protein 1
• SHC (Src homology 2 domain-containing) transforming protein 1
• SHC
• SHC1
• SHCA
• SHC-transforming protein 1
• SHC-transforming protein 3
• SHC-transforming protein A
• Src homology 2 domain-containing-transforming protein C1

Background

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc andisoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of theGRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoformp52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shcdoes not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular responseto oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and isindispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome crelease and apoptosis. The expression of isoform p66Shc has been correlated with life span

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SHC1 Antibody (NBP2-20352)(1)

Publication using NBP2-20352

• Yang YY, Deng RR, Chen Z et al. Piperazine ferulate attenuates high glucose?induced mesangial cell injury via the regulation of p66(Shc) Molecular Medicine Reports 2021-03-16 [PMID: 33760157] (Western Blot)

Bioinformatics

• Gene Symbol: SHC1