SHARPIN Antibody


Immunohistochemistry-Paraffin: SHARPIN Antibody [NBP2-27186] - Formalin-fixed, paraffin-embedded human brain stained with Sharpin antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.

Product Details

Reactivity Hu, CaSpecies Glossary
Applications WB, IHC-P
0.5 mg/ml

Order Details

SHARPIN Antibody Summary

A portion of amino acids 25-75 of human Sharpin was used as the immunogen for this antibody.
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.2 ml PBS and 0.05% BSA
0.05% Sodium Azide
0.5 mg/ml
Protein G purified


  • Western Blot 1:100-1:2000
  • Immunohistochemistry-Paraffin
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reactivity Notes

The amino acid sequence used as immunogen for the antibody is 100% homologous in human and dog, 78% in mouse and 72% homologous in rat.

Alternate Names for SHARPIN Antibody

  • DKFZp434N1923
  • hSIPL1
  • SHANK-associated RH domain interactor
  • Shank-associated RH domain-interacting protein
  • Shank-interacting protein-like 1
  • sharpin
  • SIPL1
  • SIPL1DKFZP434N1923


Sharpin (SHANk-associated RH domain interacting protein) is a 387-amino acid protein, enriched in the postsynaptic density (PSD) of excitatory neurotransmitters. It is abundantly expressed in brain, spleen, lung, heart, skeletal muscle, kidney and testis (Stieglitz, 2012). A component of the large multi-protein E3 ubiquitin ligase complex called LUBAC, Sharpin regulates cell survival and apoptosis, cytokine production, and development of lymphoid tissues by regulating TNF-alpha induced canonical NF-kappaB signaling. Sharpin binds to the LUBAC subunit HOIP, and stimulates the formation of linear ubiquitin chains in vitro and in vivo. Sharpin harbors multiple functional motifs including an amino terminal coiled-coil (CC) domain, which has been shown to mediate the interaction between Sharpin and the scaffold protein Shank. The other two domains, UBL and NPL4 zinc finger domain facilitate ubiquitin-mediated protein recognition and degradation. Sharpin also acts as an inhibitor of beta1-integrins activation and down-regulates the activity of tumor suppressor protein PTEN, thus modulating immune and inflammatory diseases (Wang, 2012).Sharpin antibody recognizes endogenous levels of total Sharpin, which is recently identified as a commonly up-regulated gene in many human cancers and is believed to have a role in tumor biogenesis. It may function in small GTPase-mediated signaling pathways that may transform the actin cytoskeleton, thereby inducing morphological changes needed for tumor metastasis (Jung, 2010). Thus, Sharpin antibody plays an important role in normal immune development and control of inflammation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Gene Symbol SHARPIN

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