HOIP/RNF31 Antibody (875227) [Unconjugated]

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human HOIP/RNF31 Monoclonal Antibody (Catalog ...read more
HOIP/RNF31 was detected in immersion fixed MCF‑7 human breast cancer cell line, untreated (upper panel) or treated with Proteasome Inhibitor I (Tocris Bioscience, Catalog # 4045, lower panel), using Mouse Anti-Human ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for HOIP/RNF31 at approximately 144 kDa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
875227
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

HOIP/RNF31 Antibody (875227) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HOIP/RNF31
Arg970-Lys1072
Accession # Q96EP0
Specificity
Detects human HOIP/RNF31 in direct ELISA and Western Blot.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RNF31
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB8039 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HOIP/RNF31 Antibody (875227) [Unconjugated]

  • EC 6.3.2.-
  • FLJ10111
  • FLJ23501
  • HOIP
  • MGC19975
  • ring finger protein 31
  • RNF31
  • ZIBRA
  • ZIBRAHOIL-1-interacting protein
  • Zinc in-between-RING-finger ubiquitin-associated domain protein

Background

RNF31 (RING [Really INteresting Gene] Finger Protein 31; also HOIL-1-interacting protein/HOIP, and Zn in-between-RING-finger ubiquitin-associated domain protein/ZIBRA) is a cytoplasmic E3 ubiquitin-protein ligase that is found in breast epithelium and multiple cancer types. Although its predicted MW is 102 kDa, it runs anomalously at 95-135 kDa in SDS-Page. Ubiquitin (Ub) chains are typically thought of as 9 kDa additions to Lys residues of target molecules. The activity associated with Ub addition depends upon the location of the attachment, and the monomeric vs. polymeric nature of the chains. Ub can also be added to N-terminal Met residues by an intracellular complex called LUBAC (Linear Ub chain Assembly Complex). This complex is key to NF kappa B pathway activation. Following exposure of cells to cytokines, LUBAC ubiquitinates NEMO, which subsequently induces IKK beta phosphorylation, I kappa B alpha degradation, and NF kappa B translocation into the nucleus with gene activation. The LUBAC complex contains RNF31, HOIL-1L and sharpin, and it is now known that RNF31 is the catalyst for linear Ub chain formation. Human RNF31 is 1072 amino acids (aa) in length. It contains three consecutive RanBP2-type Zn finger domains (aa 299-438), a utilized phosphoryation site at Ser466, one UBA domain (aa 564-615), and two RING-type Zn finger domains (aa 699-747 and 860-909) with an intervening IBR-type Zn finger region (aa 779-841). There are at least two isoform variants, one that contains a 13 aa substitution for aa 1-164, and another that shows a deletion of aa 73-630 coupled to a deletion of aa 833-841. Human RNF31 shares 87% aa sequence identity with mouse RNF31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HOIP/RNF31 Antibody (MAB8039)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry, ICC, Western Blot.


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Flow Cytometry
(1)
ICC
(1)
Western Blot
(2)
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Filter By Species
Human
(2)
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Showing Publications 1 - 8 of 8.
Publications using MAB8039 Applications Species
Zhengkui Zhang, Xiangjun Kong, Maarten A. Ligtenberg, Susan E. van Hal-van Veen, Nils L. Visser, Beaunelle de Bruijn et al. RNF31 inhibition sensitizes tumors to bystander killing by innate and adaptive immune cells Cell Reports Medicine 2022-06-09 [PMID: 35688159]
Jacquelyn R. Bedsaul, Neha Shah, Shelby M. Hutcherson, Joel L. Pomerantz Mechanistic impact of oligomer poisoning by dominant-negative CARD11 variants iScience 2022-02-18 [PMID: 35198875]
Hongli Yin, Ozge Karayel, Ying-Yin Chao, Thomas Seeholzer, Isabel Hamp, Oliver Plettenburg et al. A20 and ABIN-1 cooperate in balancing CBM complex-triggered NF-kappa B signaling in activated T cells Cellular and Molecular Life Sciences 2022-02-01 [PMID: 35099607]
Chao-Tao Tang, Jing Yang, Zi-De Liu, Youxiang Chen, Chunyan Zeng Taraxasterol acetate targets RNF31 to inhibit RNF31/p53 axis-driven cell proliferation in colorectal cancer Cell Death Discovery 2021-04-06 [PMID: 33824292]
T Douglas, M Saleh Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death Cell Rep, 2019-12-10;29(11):3652-3663.e5. 2019-12-10 [PMID: 31825842] (Western Blot, Human) Western Blot Human
F Zhu, G Yi, X Liu, F Zhu, A Zhao, A Wang, R Zhu, Z Chen, B Zhao, S Fang, X Yu, R Lin, R Liang, D Li, W Zhao, Z Zhang, W Guo, S Zhang, S Ge, X Fan, G Zhao, B Li Ring finger protein 31-mediated atypical ubiquitination stabilizes forkhead box P3 and thereby stimulates regulatory T-cell function J. Biol. Chem., 2018-11-02;0(0):. 2018-11-02 [PMID: 30389786] (Flow Cytometry, ICC, Western Blot, Human) Flow Cytometry, ICC, Western Blot Human
Maarten F. de Jong, Zixu Liu, Didi Chen, Neal M. Alto Shigella flexneri suppresses NF-kappa B activation by inhibiting linear ubiquitin chain ligation Nature Microbiology 2016-05-27 [PMID: 27572974]
Lisa Schlicher, Manuela Wissler, Florian Preiss, Prisca Brauns‐Schubert, Celia Jakob, Veronica Dumit et al. SPATA 2 promotes CYLD activity and regulates TNF ‐induced NF ‐ kappa B signaling and cell death EMBO reports 2016-10-01 [PMID: 27458237]

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Bioinformatics

Gene Symbol RNF31
Uniprot