SH3BP4 Overexpression Lysate (Native)

Images

 
Western Blot: SH3BP4 Overexpression Lysate (Adult Normal) [NBL1-15928] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SH3BP4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SH3BP4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208730 and based on accession number NM_014521. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens SH3-domain binding protein 4 (SH3BP4), mRNA.
Gene
SH3BP4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
107.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SH3BP4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SH3BP4 Overexpression Lysate (Native)

  • BOG25SH3 domain-binding protein 4
  • EHB10
  • EH-binding protein 10
  • SH3-domain binding protein 4
  • transferrin receptor trafficking protein
  • Transferrin receptor-trafficking protein
  • TTP

Background

This gene encodes a protein with 3 Asn-Pro-Phe (NPF) motifs, an SH3 domain, a PXXP motif, a bipartite nuclear targeting signal, and a tyrosine phosphorylation site. This protein is involved in cargo-specific control of clathrin-mediated endocytosis, specifically controlling the internalization of a specific protein receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol SH3BP4