Recombinant Human sFRP-5 Protein Summary
| Description |
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 30-317 of Human sFRP-5 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSEEYDYYG WQAEPLHGRS YSKPPQCLDI PADLPLCHTV GYKRMRLPNL LEHESLAEVK QQASSWLPLL AKRCHSDTQV FLCSLFAPVC LDRPIYPCRS LCEAVRAGCA PLMEAYGFPW PEMLHCHKFP LDNDLCIAVQ FGHLPATAPP VTKICAQCEM EHSADGLMEQ MCSSDFVVKM RIKEIKIENG DRKLIGAQKK KKLLKPGPLK RKDTKRLVLH MKNGAGCPCP QLDSLAGSFL VMGRKVDGQL LLMAVYRWDK KNKEMKFAVK FMFSYPCSLY YPFFYGAAEP H |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
SFRP5 |
| Purity |
>85%, by SDS-PAGE |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. |
| Theoretical MW |
35 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol |
| Preservative |
No Preservative |
| Concentration |
1 mg/ml |
| Purity |
>85%, by SDS-PAGE |
Alternate Names for Recombinant Human sFRP-5 Protein
Background
Human secreted frizzled-related protein 5 (SFRP5), which appears to act by modulating Wnt signal transduction. Wnts have been implicated in embryonic induction, cell fate determination, control of cell polarity and malignant transformation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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