6266-SF) inhibits Recombinant Mouse Wnt-3a (Catalog #
1324-WN) induced Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity inhibited by Recombinant Human sFRP-5 (5 µg/mL) is neutralized (blue line) by increasing concentrations of Mouse Anti-Human sFRP-5 Monoclonal Antibody (Catalog # MAB6266) in the presence of 100 ng/mL Recombinant Mouse Wnt-3a. The ND
50 is typically 2-10 µg/mL." class="big_lightbox">
6266-SF) inhibits Recombinant Mouse Wnt-3a (Catalog #
1324-WN) induced Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity inhibited by Recombinant Human sFRP-5 (5 µg/mL) is neutralized (blue line) by increasing concentrations of Mouse Anti-Human sFRP-5 Monoclonal Antibody (Catalog # MAB6266) in the presence of 100 ng/mL Recombinant Mouse Wnt-3a. The ND
50 is typically 2-10 µg/mL." alt="Recombinant Human sFRP-5 (Catalog #
6266-SF) inhibits Recombinant Mouse Wnt-3a (Catalog #
1324-WN) induced Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity inhibited by Recombinant Human sFRP-5 (5 µg/mL) is neutralized (blue line) by increasing concentrations of Mouse Anti-Human sFRP-5 Monoclonal Antibody (Catalog # MAB6266) in the presence of 100 ng/mL Recombinant Mouse Wnt-3a. The ND
50 is typically 2-10 µg/mL." class="big_thumb" />
6266-SF) inhibits Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induced Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity inhibited by Recombinant Human sFRP-5 (5 µg/mL) is neutralized (blue line) by increasing concentrations of Mouse Anti-Human sFRP-5 Monoclonal Antibody (Catalog # MAB6266) in the presence of 100 ng/mL Recombinant Mouse Wnt-3a. The ND50 is typically 2-10 µg/mL." alt="Recombinant Human sFRP-5 (Catalog # 6266-SF) inhibits Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induced Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity inhibited by Recombinant Human sFRP-5 (5 µg/mL) is neutralized (blue line) by increasing concentrations of Mouse Anti-Human sFRP-5 Monoclonal Antibody (Catalog # MAB6266) in the presence of 100 ng/mL Recombinant Mouse Wnt-3a. The ND50 is typically 2-10 µg/mL." class="big_thumb" />
![Western Blot: sFRP-5 Antibody [NBP3-12343] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 38 KDa.](http://images.novusbio.com/fullsize/sFRP-5-Antibody-Western-Blot-NBP3-12343-img0005.jpg "Western Blot: sFRP-5 Antibody [NBP3-12343] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 38 KDa.")
![Immunohistochemistry-Paraffin: sFRP-5 Antibody [NBP3-12343] - Mouse Small Intestine. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/sFRP-5-Antibody-Immunohistochemistry-Paraffin-NBP3-12343-img0004.jpg)
![Immunohistochemistry-Paraffin: sFRP-5 Antibody [NBP3-12343] - Mouse Testis. 1:100 dilution IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 20X magnification.](http://images.novusbio.com/fullsize/sFRP-5-Antibody-Immunohistochemistry-Paraffin-NBP3-12343-img0001.jpg)
![Immunohistochemistry-Paraffin: sFRP-5 Antibody [NBP3-12343] - Mouse Testis. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/sFRP-5-Antibody-Immunohistochemistry-Paraffin-NBP3-12343-img0002.jpg)
![Immunohistochemistry: sFRP-5 Antibody [NBP2-94775] - Analysis of mouse pancreas using sFRP-5 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/sFRP-5-Antibody-Immunohistochemistry-NBP2-94775-img0001.jpg "Immunohistochemistry: sFRP-5 Antibody [NBP2-94775] - Analysis of mouse pancreas using sFRP-5 . Blue: DAPI for nuclear staining.")
![sFRP-5 Antibody [NBP2-94775] - Analysis of various lysates, using SFRP5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp2-94775_rabbit-polyclonal-sfrp-5-antibody-western-blot-155202395125..jpg)
![Western Blot: SFRP5 Antibody [NB200-121] - Analysis of human SFRP5 in pancreas lysate with anti SFRP5 at a 1:500 dilution.](http://images.novusbio.com/fullsize/SFRP5-Antibody-Western-Blot-NB200-121-img0001.jpg "Western Blot: SFRP5 Antibody [NB200-121] - Analysis of human SFRP5 in pancreas lysate with anti SFRP5 at a 1:500 dilution.")
![Western Blot: SFRP5 Overexpression Lysate (Adult Normal) [NBP2-08048] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SFRP5.](http://images.novusbio.com/fullsize/SFRP5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08048-img0001.jpg "Western Blot: SFRP5 Overexpression Lysate (Adult Normal) [NBP2-08048] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SFRP5.")
