sFRP-5 Antibody

Images

 
Western Blot: sFRP-5 Antibody [NBP2-20331] - Lane 1: pcDNA3.1, Lane 2: sFRP-5 - Myc in 293T cells, Lane 3: Load half volume of lane 2, Lane 4: recombinant mouse sFRP-5, Lane 5: human sFRP-5. Image from a verified ...read more
Immunohistochemistry-Paraffin: sFRP-5 Antibody [NBP2-20331] - Human normal colon, using SFRP5 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: sFRP-5 Antibody [NBP2-20331] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, IHC, IHC-P, MiAr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

sFRP-5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SFRP5. The exact sequence is proprietary.
Localization
Secreted
Predicted Species
Mouse (95%), Rat (95%), Porcine (98%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SFRP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Microarray
Application Notes
Use in Microarray reported in scientific literature (PMID:28486105)
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-20331 in the following applications:

Publications
Read Publication using
NBP2-20331 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for sFRP-5 Antibody

  • frizzled-related protein 1b
  • FRP1B
  • FRP-1b
  • SARP3
  • SARP-3
  • secreted apoptosis related protein 3
  • secreted apoptosis-related protein 3
  • secreted frizzled-related protein 5
  • sFRP5
  • sFRP-5

Background

Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for sFRP-5 Antibody (NBP2-20331)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: MiAr, WB.


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MiAr
(1)
WB
(1)
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Human
(1)
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Review for sFRP-5 Antibody (NBP2-20331) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-20331:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot sFRP-5 NBP2-20331
Enlarge
5
reviewed by:
Anonymous
WB Mouse 07/10/2015
View

Summary

ApplicationWestern Blot
Sample TestedSfrp5-Myc in 293T cells, recombinant mouse SFRP5, recombinant human SFRP5 mouse white adipose tissue
SpeciesMouse
Lot40436

Blocking

Blocking Details5% milk

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionAmersham ECL Rabbit IgG HRP-linked whole Ab (from donkey)
Secondary Manufacturer Cat#NA934-100UL
Secondary Concentration1:2000

Details

Detection Notespositive negative control are shown in the picuture

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for sFRP-5 Antibody (NBP2-20331) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for sFRP-5 Antibody (NBP2-20331)

Discover related pathways, diseases and genes to sFRP-5 Antibody (NBP2-20331). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for sFRP-5 Antibody (NBP2-20331)

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Blogs on sFRP-5

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Calculators

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
07/10/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SFRP5
Uniprot