sFRP-5 Antibody

Western Blot: sFRP-5 Antibody [NBP2-20331] - Lane 1: pcDNA3.1, Lane 2: sFRP-5 - Myc in 293T cells, Lane 3: Load half volume of lane 2, Lane 4: recombinant mouse sFRP-5, Lane 5: human sFRP-5. Image submitted by a verified customer review.

Immunohistochemistry-Paraffin: sFRP-5 Antibody [NBP2-20331] - Human normal colon, using SFRP5 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: sFRP-5 Antibody [NBP2-20331] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:500.

Product Discontinued

sFRP-5 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SFRP5. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Mouse (95%), Rat (95%), Porcine (98%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SFRP5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Microarray
  • Western Blot 1:500-1:3000
• Application Notes: Use in Microarray reported in scientific literature (PMID:28486105). sFRP-5 antibody validated for WB from a verified customer review.
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for sFRP-5 Antibody

• frizzled-related protein 1b
• FRP1B
• FRP-1b
• SARP3
• SARP-3
• secreted apoptosis related protein 3
• secreted apoptosis-related protein 3
• secreted frizzled-related protein 5
• sFRP5
• sFRP-5

Background

Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for sFRP-5 Antibody (NBP2-20331)(1)

Publication using NBP2-20331

• Xia Limin, Huang Wenjie, Bellani Marina et al. CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell 2017-09-06 [PMID: 28486105] (WB, MiAr, Human)

Review for sFRP-5 Antibody (NBP2-20331) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 07/10/2015

Summary

• Application: Western Blot
• Sample Tested: Sfrp5-Myc in 293T cells, recombinant mouse SFRP5, recombinant human SFRP5 mouse white adipose tissue
• Species: Mouse
• Lot: 40436

Bioinformatics

• Gene Symbol: SFRP5
• Uniprot:
  • Q5T4F7()