sFRP-4 Antibody

Western Blot: sFRP-4 Antibody [NBP1-36985] - Western blot analysis of SFRP4 in Human Uterus lysate (35ug protein in RIPA buffer) using NBP1-36985 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-36985] - Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

sFRP-4 Antibody Summary

• Description: Novus Biologicals Goat sFRP-4 Antibody (NBP1-36985) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-sFRP-4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence TNSSCQCPHILPHQD corresponding to internal region according to NP_003005.2.
• Epitope: internal region
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SFRP4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in human uterus lysates (calculated MW of 39.8 kDa band according to NP_003005.2). IHC-P: Human uterus shows granular staining in the apical cytoplasm of the endometrial gland.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for sFRP-4 Antibody

• DDC-4
• Frizzled protein, human endometrium
• FRP-4
• FRP-AP
• frpHE
• FRPHEMGC26498
• FrzB2
• secreted frizzled-related protein 4
• secreted frizzled-related protein 4; secreted frizzled-related protein 4
• sFRP4
• sFRP-4

Background

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for sFRP-4 Antibody (NBP1-36985)(1)

Publication using NBP1-36985

• Linhart HG, Lin H, Yamada Y et al. Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev 2007-12-01 [PMID: 18056424]

Bioinformatics

• Gene Symbol: SFRP4
• Entrez:
  • 20379(Mouse)
  • 89803(Rat)
• Uniprot:
  • NP_003005.2(Human)