sFRP-3/FRZB Antibody [Unconjugated]

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and PANC-1 human pancreatic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse sFRP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF192) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for sFRP-3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

sFRP-3/FRZB Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human sFRP-3 (R&D Systems, Catalog # 192-SF)
  Ala33-Asn325
  Accession # AAB51298
• Specificity: Detects human and mouse sFRP-3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FRZB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1-1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for sFRP-3/FRZB Antibody [Unconjugated]

• FIZ
• FREOS1
• Frezzled
• Fritz
• Frizzled-related protein 1
• frizzled-related protein
• FRP
• FRP-3
• FRZB
• Frzb1
• FRZB-1
• FRZB1sFRP-3
• FRZB-PEN
• FZRB
• hFIZ
• secreted frizzled-related protein 3
• sFRP3
• sFRP-3
• SFRP3frezzled
• SRFP3frizzled homolog-related

Background

Secreted Frizzled Related Protein 3 (sFRP-3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. SFRP-3 is often referred to as FRZB, other names include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, sFRP-3 is highly conserved. The human protein shares 77% identity with Xenopus, 92% with mouse, and 94% with bovine proteins. Human sFRP-3 is strongly expressed in the developing appendicular skeleton, and cartilage of craniofacial bones. As determined by Northern blot of adult tissues, it is strongly detected in heart and placenta, as well as the brain, skeletal muscle, kidney and pancreas.

The N-terminal portion of the human protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of sFRP-3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. SFRP-3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of sFRP-3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA.

1. Hoang, et al. (1996) J. Biol. Chem. 271:26131.
2. Leyns, et al. (1997) Cell 88:747.
3. Wang, et al. (1997) Cell 88:757.
4. Mayr, et al. (1997) Mech. Dev. 63:109.
5. Rattner, et al. (1997) PNAS 94:2859.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for sFRP-3/FRZB Antibody (AF192)(4)

Publications using AF192

• MT De Angelis, EI Parrotta, G Santamaria, G Cuda Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells Cell Death Dis, 2018-01-05;9(1):6. 1/5/2018 [PMID: 29305588]
• Jingjing You, Li Wen, Athena Roufas, Michele C. Madigan, Gerard Sutton Expression of SFRP Family Proteins in Human Keratoconus Corneas PLoS ONE 6/18/2013 [PMID: 23825088]
• Lane NE, Lian K, Nevitt MC, Zmuda JM, Lui L, Li J, Wang J, Fontecha M, Umblas N, Rosenbach M, de Leon P, Corr M Frizzled-related protein variants are risk factors for hip osteoarthritis. Arthritis Rheum., 2006-04-01;54(4):1246-54. 2006-04-01 [PMID: 16572458] (ELISA Development, Human)
• Loughlin J, Dowling B, Chapman K, Marcelline L, Mustafa Z, Southam L, Ferreira A, Ciesielski C, Carson DA, Corr M Functional variants within the secreted frizzled-related protein 3 gene are associated with hip osteoarthritis in females. Proc. Natl. Acad. Sci. U.S.A., 2004-06-21;101(26):9757-62. 2004-06-21 [PMID: 15210948] (IHC-Fr, Western Blot, Human)

Bioinformatics

• Gene Symbol: FRZB
• Uniprot:
  • AAB51298()