FRZB Antibodies

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="sFRP-3 was detected in immersion fixed frozen sections of mouse embryo (E15) using Mouse sFRP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF592) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF017). A specific band was detected for sFRP-3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for sFRP-3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and PANC-1 human pancreatic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse sFRP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF192) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for sFRP-3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

192-SF) was serially diluted and captured by Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB1921) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB192) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 192-SF) was serially diluted and captured by Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB1921) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB192) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human sFRP‑3 (Catalog # 192-SF) was serially diluted and captured by Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB1921) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB192) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

192-SF) was serially diluted and captured by Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB1921) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB192) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 192-SF) was serially diluted and captured by Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB1921) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB192) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human sFRP‑3 (Catalog # 192-SF) was serially diluted and captured by Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB1921) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB192) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />