sFRP-1 Antibody

Images

 
Immunohistochemistry-Paraffin: sFRP-1 Antibody [NBP1-51926] - IHC staining of paraffin embedded human kidney (3.8ug/ml). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

sFRP-1 Antibody Summary

Immunogen
Peptide with sequence KNHECPTFQSVFK corresponding to C-Terminus according to NP_003003.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SFRP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:1000
Application Notes
IHC-P: Human kidney shows vesicular staining in epithelial cells of PCT.
Publications
Read Publication using NBP1-51926.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for sFRP-1 Antibody

  • FRP1
  • FRP-1FrzA
  • FRPSARP-2
  • FrzA
  • SARP-2
  • SARP2sFRP-1
  • Secreted apoptosis-related protein 2
  • secreted frizzled-related protein 1
  • sFRP1
  • sFRP-1

Background

SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzledrelated Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for sFRP-1 Antibody (NBP1-51926)(1)

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Product General Protocols

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FAQs for sFRP-1 Antibody (NBP1-51926). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in this SFRP1 Antibody (NBP1-51926). On its application, it said it can be used in ELISA and the peptide ELISA dilution 1:4000. I am a bit confused on the statement. I don't have the peptide, so won't use this antibody in any peptide ELISA. Has this been tested in a normal ELISA such as sandwich ELISA?
    • This product has only been tested in Elisa for detection of the peptide that is also available for purchase. However, I have found it has also been validated in IHC, so it should work in a standard sandwich ELISA to detect the endogenous protein and we would guarantee this.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SFRP1
Entrez
Uniprot