sFRP-1 Antibody [Unconjugated]

sFRP-1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human sFRP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1384) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

sFRP-1 was detected in immersion fixed MBA-MB-468 human breast cancer cell line using Goat Anti-Human sFRP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1384) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

sFRP-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human sFRP-1 (R&D Systems, Catalog # 1384-SF)
  Ser32-Lys314
  Accession # AAB70793
• Specificity: Detects human sFRP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) sFRP-2, and rhsFRP-5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SFRP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for sFRP-1 Antibody [Unconjugated]

• FRP1
• FRP-1FrzA
• FRPSARP-2
• FrzA
• SARP-2
• SARP2sFRP-1
• Secreted apoptosis-related protein 2
• secreted frizzled-related protein 1
• sFRP1
• sFRP-1

Background

Secreted Frizzled Related Proteins (sFRPs) are a family of secreted, soluble vertebrate glycoproteins which contain homology to the Wnt-binding domain of the Frizzled (Fz) family of transmembrane receptors. sFRPs are approximately 30-35 kDa in size and are comprised of 3 domains: a signal sequence; an N-terminal Fz cysteine-rich domain (CRD) with 10 conserved cysteines; and a C-terminal heparin-binding region with weak homology to Netrin. The Fz CRD mediates Wnt-binding and is present in all Fz and sFRP family members (1).

sFRP-1, also known as secreted apoptosis-related protein 2 (SARP-2), FRP and FrzA, is expressed in the embryonic kidney, eye, brain, teeth, salivary gland and small intestine, most often at sites of epithelial-mesenchyme interaction (5). Expression in the adult animal is strong in the eye, kidney, and heart and also prevalent in the brain and lung (2, 5). sFRP-1 was first characterized as a protein that enhances the sensitivity of cells to apoptotic stimuli (3) and as an antagonist of Wnt signaling in Xenopus embryos (4). It is also characterized as a tumor suppressor in breast (6) and cervical carcinomas (7). In contrast, sFRP-1 is expressed by the majority of malignant gliomas (8) and contributes to the development of uterine leiomyomas (9), suggesting that the role of sFRP-1 is dependent on cell context. sFRP-1 has diverse activities, from inducing angiogenesis (10) in a variety of in vivo models to helping regulate Wnt-4 signaling (with sFRP-2) in renal organogenesis (11). Mouse and human sFRP-1 proteins share 94% amino acid identity (1).

1. Jones, S. et al. (2002) Bioessays 24:811.
2. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2859.
3. Melkonyan, H. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13636.
4. Finch, P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6770.
5. Leimeister, C. et al. (1998) Mech. Dev. 75:29.
6. Ugolini, F. et al. (2001) Oncogene 20:5810.
7. Ko, J. et al. (2002) Exp. Cell. Res. 280:280.
8. Roth, W. et al. (2000) Oncogene 19:4210.
9. Fukuhara, K, et al. (2002) J. Clin. Endocr. Metab. 87:1729.
10. Dufourcq, P. et al. (2002) Circulation 106: 3097.
11. Yoshino, K. et al. (2001) Mech. Dev. 102:45.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for sFRP-1 Antibody (AF1384)(8)

Publications using AF1384

• Ayoubi, R;Southern, K;Laflamme, C; A guide to selecting high-performing antibodies for Secreted frizzled-related protein 1 (sFRP-1) for use in Western Blot and immunoprecipitation F1000Research 2024-04-02 [PMID: 39319244] (Immunoprecipitation, Western Blot, Human)
• M Pehlivan, C Caliskan, Z Yuce, HO Sercan Forced expression of Wnt antagonists sFRP1 and WIF1 sensitizes chronic myeloid leukemia cells to tyrosine kinase inhibitors Tumour Biol., 2017-05-01;39(5):1010428317701. 2017-05-01 [PMID: 28468589] (Western Blot, Human)
• Low expression of RBMS3 and SFRP1 are associated with poor prognosis in patients with gastric cancer Am J Cancer Res, 2016-11-01;6(11):2679-2689. 2016-11-01 [PMID: 27904780] (IHC-P, Western Blot, Human)
• Schummer M, Thorpe J, Giraldez M et al. Evaluating Serum Markers for Hormone Receptor-Negative Breast Cancer. PLoS One. 11/13/2015 [PMID: 26565788]
• Hu J, Dong A, Fernandez-Ruiz V, Shan J, Kawa M, Martinez-Anso E, Prieto J, Qian C Blockade of Wnt signaling inhibits angiogenesis and tumor growth in hepatocellular carcinoma. Cancer Res., 2009-08-18;69(17):6951-9. 2009-08-18 [PMID: 19690140] (Western Blot, Human)
• Gogolla N, Galimberti I, Deguchi Y, Caroni P Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus. Neuron, 2009-05-28;62(4):510-25. 2009-05-28 [PMID: 19477153] (IHC-Fr, Mouse)
• Lane NE, Nevitt MC, Lui LY, de Leon P, Corr M Wnt signaling antagonists are potential prognostic biomarkers for the progression of radiographic hip osteoarthritis in elderly Caucasian women. Arthritis Rheum., 2007-10-01;56(10):3319-25. 2007-10-01 [PMID: 17907185] (ELISA Development, Human)
• Thompson MC, Fuller C, Hogg TL, Dalton J, Finkelstein D, Lau CC, Chintagumpala M, Adesina A, Ashley DM, Kellie SJ, Taylor MD, Curran T, Gajjar A, Gilbertson RJ Genomics identifies medulloblastoma subgroups that are enriched for specific genetic alterations. J. Clin. Oncol., 2006-03-27;24(12):1924-31. 2006-03-27 [PMID: 16567768] (IHC, Human)

Review for sFRP-1 Antibody (AF1384) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Cathy Guo IF Mouse 10/22/2020

Summary

• Application: Immunofluorescence
• Sample Tested: Frozen section from 4% PFA fixed mouse brain.
• Species: Mouse
• Lot: IRQ1018091

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: SFRP1
• Uniprot:
  • AAB70793()