SF3B4 Antibody

Images

 
Western Blot: SF3B4 Antibody [NB100-1076] - Western blot analysis of SF3B4 in Hela lysate using NB100-1076 at 0.1 ug/ml.
Western Blot: SF3B4 Antibody [NB100-1076] - staining (0.1ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SF3B4 Antibody Summary

Immunogen
Peptide with sequence AAGPISERNQDAT-C corresponding to N-Terminus according to NP_005841.1.
Localization
Nuclear
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SF3B4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 48 kDa band observed in human HeLa lysates (calculated MW of 44.4 kDa band according to NP005841.1).
Control
HeLa Whole Cell Lysate
Publications
Read Publication using NB100-1076.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SF3B4 Antibody

  • Hsh49
  • pre-mRNA splicing factor SF3b 49 kDa subunit
  • Pre-mRNA-splicing factor SF3b 49 kDa subunit
  • SAP 49
  • SAP49MGC10828
  • SF3b49
  • SF3b50
  • spliceosomal protein
  • spliceosome-associated protein (U2 snRNP)
  • Spliceosome-associated protein 49
  • splicing factor 3B subunit 4
  • splicing factor 3b, subunit 4, 49kD
  • splicing factor 3b, subunit 4, 49kDa

Background

The SF3B4 gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-79848
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP2-75656
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-53095
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13920
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-87431
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-75630
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB200-106
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-57487
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-82454
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1077
Species: Hu
Applications: WB, ELISA, IHC, IHC-Fr, PEP-ELISA
H00010263-B01P
Species: Hu, Mu, Rt
Applications: WB
NBP1-71687
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NB110-89726
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-16035
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-80965
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00010907-M01
Species: Hu
Applications: WB, ELISA, S-ELISA
NBP1-84718
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1936
Species: Hu, Mu, Rt, Pm, Xp, Ze
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
H00006607-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
NB100-1076
Species: Hu, Mu, Rt, Po, Bv, Ca
Applications: WB, PEP-ELISA

Publications for SF3B4 Antibody (NB100-1076)(1)

Reviews for SF3B4 Antibody (NB100-1076) (0)

There are no reviews for SF3B4 Antibody (NB100-1076). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SF3B4 Antibody (NB100-1076) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional SF3B4 Products

Array NB100-1076

Bioinformatics Tool for SF3B4 Antibody (NB100-1076)

Discover related pathways, diseases and genes to SF3B4 Antibody (NB100-1076). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SF3B4 Antibody (NB100-1076)

Discover more about diseases related to SF3B4 Antibody (NB100-1076).
 

Pathways for SF3B4 Antibody (NB100-1076)

View related products by pathway.

PTMs for SF3B4 Antibody (NB100-1076)

Learn more about PTMs related to SF3B4 Antibody (NB100-1076).

Blogs on SF3B4

There are no specific blogs for SF3B4, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SF3B4 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SF3B4
Entrez
Uniprot