SF20/MYDGF Antibody (2D4) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SF20/MYDGF Antibody (2D4) [H00056005-M03] - Analysis of monoclonal antibody to SF20/MYDGF on HeLa cell. Antibody concentration 10 ug/ml

ELISA: SF20/MYDGF Antibody (2D4) [H00056005-M03] - Detection limit for recombinant GST tagged SF20/MYDGF is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: ELISA, ICC/IF
• Clone: 2D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SF20/MYDGF Antibody (2D4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SF20/MYDGF Antibody (2D4) - Azide and BSA Free (H00056005-M03) is a monoclonal antibody validated for use in ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: SF20/MYDGF (AAH10129, 1 a.a. ~ 173 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAPSGGWNGVGASLWAALLLGAVALRPAEAVSEPTTVAFDVRPGGVVHSFSHNVGPGDKYTCMFTYASQGGTNEQWQMSLGTSEDHQHFTCTIWRPQGKSYLYFTQFKAEVRGAEIEYAMAYSKAAFERESDVPLKTEEFEVTKTAVAHRPGAFKAELSKLVIVAKASRTEL
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYDGF
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1ng/mL
  • Immunocytochemistry/ Immunofluorescence 10ug/mL
• Application Notes: Antibody reactive against recombinant protein on ELISA and Immunoflourescence. GST alone used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SF20/MYDGF Antibody (2D4) - Azide and BSA Free

• C19orf10
• chromosome 19 open reading frame 10
• EUROIMAGE1875335
• IL25
• IL27
• IL27w
• interleukin 27 working designation
• interleukin-25
• Ly6elg
• MYDGF
• myeloid-derived growth factor
• R33729_1
• SF20
• Stromal cell-derived growth factor SF20
• UPF0556 protein C19orf10

Background

Myeloid-Derived Growth Factor, or MYDGF, is a Bone marrow-derived monocyte protein, and it is correlated with enhanced metabolic activity, suppression of apoptosis, and stimulation of cell proliferation (1). MYDGF is expressed predominantly in inflammatory cells, such as monocytes and macrophages (1). Up-regulation of MYDGF expression was also found during adipocyte differentiation (2). Expression of MYDGF was induced in the circulation and heart tissue after myocardial infarction. It promotes cardiac myocyte survival by stimulating endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway, and inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (1). MYDGF was found over-expressed in approximately two-thirds of Hepatocellular Carcinoma (HCC) tissues, and its expression was significantly positively correlated with that of alpha-fetoprotein (AFP) (3). In HCC, MYDGF could regulate cell proliferation through activating Akt/mitogen-activated protein kinase pathways (3). Mouse MYDGF shares 92% amino acid sequence identity with both human and rat MYDGF. Intriguingly, virtually all homologs of MYDGF have a C-terminal putative ER retention sequence BXEL (B: Arg, His, or Lys; X: variable residue; E: Glu; L: Leu), which has the potential to retain human MYDGF and its homologs in the ER, whereas truncated MYDGF without BXEL is secreted from the cell (4). However, the functions of these different forms remain unclear.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for SF20/MYDGF Antibody (H00056005-M03). (Showing 1 - 1 of 1 FAQ).

1. Can IL 25 antibody be used in IP methods?
   • H00056005-M03 has only been validated for ELISA and Immunoflourescence in Human. If you would be interested in using this antibody for IP I invite you to read about our Innovators Reward Program.

Bioinformatics

• Gene Symbol: MYDGF
• Entrez:
  • 56005(Human)
• OMIM:
  • 606746(Human)
• Uniprot:
  • AAH10129(Human)