SETDB2 Antibody - BSA Free

Western Blot: SETDB2 Antibody [NBP2-20323] - Sample (30 ug of whole cell lysate) A: SK-N-SH 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NBP2-20323] - HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETDB2 protein stained by SETDB2 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunoprecipitation: SETDB2 Antibody [NBP2-20323] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of SETDB2 protein by 2 ug of SETDB2 antibody 7.5% SDS-PAGE The immunoprecipitated SETDB2 protein was detected by SETDB2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SETDB2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SETDB2. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SETDB2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SETDB2 Antibody - BSA Free

• C13orf4
• chromosome 13 open reading frame 4
• chronic lymphocytic leukemia deletion region 8
• Chronic lymphocytic leukemia deletion region gene 8 protein
• CLLD8DKFZp761J1217
• DKFZp586I0123
• EC 2.1.1.43
• histone-lysine N-methyltransferase SETDB2
• KMT1FCLLL8
• Lysine N-methyltransferase 1F
• SET domain bifurcated 2
• SET domain, bifurcated 2

Background

Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 [PubMed 12754510]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SETDB2