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![Western Blot: SETDB2 Antibody [NB100-1137] - Staining of Nuclear HeLa lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SETDB2-Antibody-Western-Blot-NB100-1137-img0010.jpg "Western Blot: SETDB2 Antibody [NB100-1137] - Staining of Nuclear HeLa lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NB100-1137] - Human dermal microvascular endothelial cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% TritonX-100 and immunostained with NB100-1137 (1:100 dilution). Secondary antibody conjugated to Alexafluor 488. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SETDB2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1137-img0007.jpg)
![Chromatin Immunoprecipitation (ChIP): SETDB2 Antibody [NB100-1137] - Human B-cell nuclear extract (500 ug). Gene of interest promoter primers were used for amplification to detect ChIP efficiency to IgG control. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SETDB2-Antibody-Chromatin-Immunoprecipitation-ChIP-NB100-1137-img0009.jpg)
![Western Blot: SETDB2 Antibody [NB100-1137] - RL human non-Hodgkin's lymphoma B cell line whole cell lysate (WCL). Single band around correct MW is specific to the target protein SETDB2. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SETDB2-Antibody-Western-Blot-NB100-1137-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NB100-1137] - SETDB2 in human induced Pluripotent Stem Cells (iPSCs) using anti-SETDB2 antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SETDB2-Antibody-Chromatin-Immunoprecipitation-NB100-1137-img0006.jpg)
![Western Blot: SETDB2 Antibody (2F4) [H00083852-M17] - Analysis of SETDB2 expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/SETDB2-Antibody-2F4-Western-Blot-H00083852-M17-img0001.jpg "Western Blot: SETDB2 Antibody (2F4) [H00083852-M17] - Analysis of SETDB2 expression in A-431 (Cat # L015V1).")
![Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NBP2-57394] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/SETDB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57394-img0001.jpg "Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NBP2-57394] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.")
![Western Blot: SETDB2 Antibody [NBP3-38422] - Western blot analysis of various lysates using SETDB2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38422_rabbit-setdb2-pab-912202414112827.jpg "Western Blot: SETDB2 Antibody [NBP3-38422] - Western blot analysis of various lysates using SETDB2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![SETDB1 antibody [N2C1], Internal detects SETDB1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.SETDB1 stained by SETDB1 antibody [N2C1], Internal (NBP2-20322) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20322_rabbit-polyclonal-setdb1-antibody-immunohistochemistry-paraffin-2611202518434518.jpg "SETDB1 antibody [N2C1], Internal detects SETDB1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.SETDB1 stained by SETDB1 antibody [N2C1], Internal (NBP2-20322) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry: SETDB1 Antibody [NBP2-20322] - Paraformaldehyde Fixed Human Dermal Microvascular Endothelial Cells were immunostained with 1:100 dilution of pAb. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SETDB1-Antibody-Immunocytochemistry-NBP2-20322-img0006.jpg)
![HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N2C1], Internal (NBP2-20322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20322_rabbit-polyclonal-setdb1-antibody-western-blot-2611202518411312.jpg)
![SETDB1 antibody [N2C1], Internal detects SETDB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SETDB1 stained by SETDB1 antibody [N2C1], Internal (NBP2-20322) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-20322_rabbit-polyclonal-setdb1-antibody-immunocytochemistry-immunofluorescence-261120251971821.jpg)
![SETDB1 antibody [N2C1], Internal immunoprecipitates SETDB1 protein in IP experiments.IP samples: HeLa nuclear extractA. Control with 4 ug of preimmune Rabbit IgGB. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N2C1], Internal (NBP2-20322)5 % SDS-PAGEThe immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N2C1], Internal (NBP2-20322) diluted at 1:1000.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-20322_rabbit-polyclonal-setdb1-antibody-immunoprecipitation-2611202518411320.jpg)
![Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (NBP2-20321) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20321_rabbit-polyclonal-setdb1-antibody-western-blot-2611202518292615.jpg "Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (NBP2-20321) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![HeLa whole cell and nuclear extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (NBP2-20321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20321_rabbit-polyclonal-setdb1-antibody-western-blot-2611202518342610.jpg)
![SETDB1 antibody [N1], N-term immunoprecipitates SETDB1 protein in IP experiments.IP samples: HeLa nuclear extractA. Control with 4 ug of preimmune Rabbit IgGB. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N1], N-term (NBP2-20321)5 % SDS-PAGEThe immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N1], N-term (NBP2-20321) diluted at 1:1000.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-20321_rabbit-polyclonal-setdb1-antibody-immunoprecipitation-261120251827011.jpg)
![Western Blot: SETDB1 Antibody (HL1645) - Azide and BSA Free [NBP3-25679] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL1645] (NBP3-25679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25679_rabbit-setdb1-mab-hl1645-azide-and-bsa-free-2120249355513.jpg "Western Blot: SETDB1 Antibody (HL1645) - Azide and BSA Free [NBP3-25679] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL1645] (NBP3-25679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Western Blot: SETDB1 Antibody (HL1645) - Azide and BSA Free [NBP3-25679] - HeLa whole cell and nuclear extract (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with SETDB1 antibody [HL1645] (NBP3-25679) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25679_rabbit-setdb1-mab-hl1645-azide-and-bsa-free-2120249362758.jpg)
![Immunohistochemistry-Paraffin: SETDB1 Antibody (HL1645) - Azide and BSA Free [NBP3-25679] - SETDB1 antibody [HL1645] detects SETDB1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. SETDB1 stained by SETDB1 antibody [HL1645] (NBP3-25679) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25679_rabbit-setdb1-mab-hl1645-azide-and-bsa-free-212024940855.jpg)
![Western Blot: SETDB1 Antibody (HL1645) - Azide and BSA Free [NBP3-25679] - Various tissue extracts (50 Aug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL1645] (NBP3-25679) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25679_rabbit-setdb1-mab-hl1645-azide-and-bsa-free-2120249403821.jpg)
![Immunocytochemistry/Immunofluorescence: SETDB1 Antibody (HL1645) - Azide and BSA Free [NBP3-25679] - SETDB1 antibody [HL1645] detects SETDB1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETDB1 stained by SETDB1 antibody [HL1645] (NBP3-25679) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25679_rabbit-setdb1-mab-hl1645-azide-and-bsa-free-212024941495.jpg)
![Western Blot: SETDB1 Antibody [NBP1-77990] - detect human ESET present in a 293 whole cell lysate. ~30ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min.](http://images.novusbio.com/fullsize/SETDB1-Antibody-Western-Blot-NBP1-77990-img0001.jpg "Western Blot: SETDB1 Antibody [NBP1-77990] - detect human ESET present in a 293 whole cell lysate. ~30ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min.")
![Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL3260] (NBP3-48557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48557_rabbit-setdb1-mab-hl3260-western-blot-1411202518363624.jpg "Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL3260] (NBP3-48557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![SETDB1 antibody [HL3260] detects SETDB1 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SETDB1 stained by SETDB1 antibody [HL3260] (NBP3-48557) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-48557_rabbit-setdb1-mab-hl3260-immunocytochemistry-immunofluorescence-141120251837274.jpg)
![<b>Orthogonal Strategies Validation. </b>HeLa whole cell and nuclear extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL3260] (NBP3-48557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48557_rabbit-setdb1-mab-hl3260-western-blot-14112025183378.jpg)
![Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL3259] (NBP3-48783) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48783_rabbit-setdb1-mab-hl3259-western-blot-1411202518294113.jpg "Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL3259] (NBP3-48783) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![HeLa whole cell and nuclear extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL3259] (NBP3-48783) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48783_rabbit-setdb1-mab-hl3259-western-blot-1411202518354613.jpg)
![<b>Orthogonal Strategies Validation. </b>SETDB1 antibody [HL3259] detects SETDB1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.SETDB1 stained by SETDB1 antibody [HL3259] (NBP3-48783) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA expression data for the same tissues are based on NCBI.](http://images.novusbio.com/fullsize/nbp3-48783_rabbit-setdb1-mab-hl3259-immunohistochemistry-paraffin-1411202518382817.jpg)
![Western Blot: SETDB1 Antibody (5H6A12) [NBP1-51677] - Analysis using SETDB1 mouse mAb against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5), F9 (6), RAW246.7 (7) and Cos7 (8) cell lysate.](http://images.novusbio.com/fullsize/SETDB1-Antibody-5H6A12-Western-Blot-NBP1-51677-img0007.jpg "Western Blot: SETDB1 Antibody (5H6A12) [NBP1-51677] - Analysis using SETDB1 mouse mAb against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5), F9 (6), RAW246.7 (7) and Cos7 (8) cell lysate.")
![Immunocytochemistry/Immunofluorescence: SETDB1 Antibody (5H6A12) [NBP1-51677] - Analysis of LOVO cells using SETDB1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/SETDB1-Antibody-5H6A12-Immunocytochemistry-Immunofluorescence-NBP1-51677-img0005.jpg)