SET Antibody

Images

 
Western Blot: SET Antibody [NBP1-33713] - Western blot analysis of SET on Molt-4 lysates using NBP1-33713 at a dilution of 1:1000. 30 ug whole cell lysate loaded on 12% SDS PAGE gel.
Immunocytochemistry/ Immunofluorescence: SET Antibody [NBP1-33713] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SET (NBP1-33713) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SET Antibody [NBP1-33713] - Immunohistochemical analysis of SET on paraffin-embedded DLD-1 xenograft, using NBP1-33713.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.43 mg/ml

Order Details

SET Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SET. The exact sequence is proprietary.
Localization
Cytoplasm, cytosol, Endoplasmic reticulum, Nucleus, nucleoplasm
Specificity
Antibody reactive against recombinant protein, detects immunogen. Further validation in progress.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SET
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100-1:500

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.43 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SET Antibody

  • 2PP2A
  • HLA-DR-associated protein II
  • I2PP2A
  • I-2PP2A
  • IGAAD
  • Inhibitor of granzyme A-activated DNase
  • inhibitor-2 of protein phosphatase-2A
  • IPP2A2
  • PHAPIITAF-I
  • Phosphatase 2A inhibitor I2PP2A
  • protein phosphatase type 2A inhibitor
  • protein SET
  • SET nuclear oncogene
  • SET translocation (myeloid leukemia-associated)
  • TAF-IBETA
  • Template-activating factor I
  • Template-Activating Factor-I, chromatin remodelling factor

Background

SET belongs to a family of multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. There are two named isoforms produced by alternative splicing : Isoform 1 and Isoform 2. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional SET Products

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Diseases for SET Antibody (NBP1-33713)

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Pathways for SET Antibody (NBP1-33713)

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PTMs for SET Antibody (NBP1-33713)

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Bioinformatics

Gene Symbol SET
Entrez
Uniprot