SET Antibody

Western Blot: SET Antibody [NBP1-26646] - Detection of Human and Mouse SET by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SET antibody NBP1-26646 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: SET Antibody [NBP1-26646] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- SET used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Western Blot: SET Antibody [NBP1-26646] - Whole cell lysate from HeLa and 293T cells. SET was also immunoprecipitated by rabbit anti-SET antibody NBP1-26647.

Immunohistochemistry: SET Antibody [NBP1-26646] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SET used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SET Antibody Summary

• Description: Novus Biologicals Rabbit SET Antibody (NBP1-26646) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human SET nuclear oncogene using the numbering given in entry NP_001116293.1 (GeneID 6418).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SET
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 3-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SET Antibody

• 2PP2A
• HLA-DR-associated protein II
• I2PP2A
• I-2PP2A
• IGAAD
• Inhibitor of granzyme A-activated DNase
• inhibitor-2 of protein phosphatase-2A
• IPP2A2
• PHAPIITAF-I
• Phosphatase 2A inhibitor I2PP2A
• protein phosphatase type 2A inhibitor
• protein SET
• SET nuclear oncogene
• SET translocation (myeloid leukemia-associated)
• TAF-IBETA
• Template-activating factor I
• Template-Activating Factor-I, chromatin remodelling factor

Background

SET is a multi-functional protein involved in apoptosis, transcription, nucleosome assembly, and histone binding. SET was identified as part of a chromosomal rearrangement with NUP214/CAN in acute undifferentiated leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SET
• Entrez:
  • 6418(Human)
• Uniprot:
  • Q01105(Human)