Recombinant Human Serum Amyloid A1 Protein Summary
| Description |
Recombinant bioactive protein containing 18 amino acids for Human Serum Amyloid A1 Source:E. coli Amino Acid Sequence:(P0DJI8) |
Preparation Method |
Determined to be >98% pure by SDS-PAGE |
| Details of Functionality |
For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 ug/ml. |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
SAA1 |
| Purity |
>98%, by SDS-PAGE |
| Endotoxin Note |
<0.1 ng/ug |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
11.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer |
Lyophilized with no additives. |
| Concentration |
Lyoph |
| Purity |
>98%, by SDS-PAGE |
| Reconstitution Instructions |
Reconstitute in 0.1% acetic acid to 1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage. |
Alternate Names for Recombinant Human Serum Amyloid A1 Protein
Background
Human apo-SAA is a 104 amino acid polypeptide that circulates primarily in association with high-density lipoproteins (HDL). The level of apo-SAA, normally 1-5ug/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL apo-lipoprotein. The human SAA gene codes for a 122 amino acid polypeptide, which contains an 18 amino acid N-terminal signal sequence.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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