Recombinant Human SerpinB2 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Format
Carrier-Free
Concentration
LYOPH

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Catalog# & Formulation Size Price

Recombinant Human SerpinB2 Protein Summary

Description
A biologically active protein to SERPINB2.

Source: E. coli

Amino Acid Sequence: MEDLCVANTL FALNLFKHLA KASPTQNLFL SPWSISSTMA MVYMGSRGST EDQMAKVLQF NEVGANAVTP MTPENFTSCG FMQQIQKGSY PDAILQAQAA DKIHSSFRSL SSAINASTGN YLLESVNKLF GEKSASFREE YIRLCQKYYS SEPQAVDFLE CAEEARKKIN SWVKTQTKGK IPNLLPEGSV DGDTRMVLVN AVYFKGKWKT PFEKKLNGLY PFRVNSAQRT PVQMMYLREK LNIGYIEDLK AQILELPYAG DVSMFLLLPD EIADVSTGLE LLESEITYDK LNKWTSKDKM AEDEVEVYIP QFKLEEHYEL RSILRSMGME DAFNKGRANF SGMSERNDLF LSEVFHQAMV DVNEEGTEAA AGTGGVMTGR TGHGGPQFVA DHPFLFLIMH KITNCILFFG RFSSP
Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
SerpinB2 Protein is fully biologically active when compared to standard. The biologically active was determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37 degrees C. Half maximal inhibition against 1.0 ug/ml of single chain tPA was at a concentration of 1.0 ug/ml.
Protein/Peptide Type
Biologically Active Protein
Gene
SERPINB2
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 0.1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
46.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM Cysteine, with 5 % Trehalose.
Preservative
5% Trehalose
Concentration
LYOPH
Purity
>95% pure by SDS-PAGE
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human SerpinB2 Protein

  • HsT1201
  • Monocyte Arg-serpin
  • PAI 2
  • PAI
  • PAI2
  • PAI-2
  • PAI2Urokinase inhibitor
  • Placental plasminogen activator inhibitor
  • PLANH2
  • PLANH2PAI-2
  • plasminogen activator inhibitor 2
  • plasminogen activator inhibitor, type II (arginine-serpin)
  • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2
  • Serpin B2
  • serpin peptidase inhibitor, clade B (ovalbumin), member 2
  • Urokinase Inhibitor

Background

SerpinB2 (PAI-2) is an inhibitory serpin expressed mainly in keratinocytes, activated monocytes, and placental trophoblasts. It exists predominantly as a 47 kDa non-glycosylated intracellular protein which can be induced to be secreted as 60 kDa glycoprotein. The glycosylated and unglycosylated forms of SerpinB2 (PAI-2) are equally effective as inhibitors of urokinase-type plasminogen activator (uPA), the only established physiological target of this serpin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol SERPINB2
Entrez
Uniprot