Serpin I2 Antibody

Western Blot: Serpin I2 Antibody [NB100-1782] - Staining of Human Pancreas lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Serpin I2 Antibody [NB100-1782] - Staining of paraffin embedded Human Pancreas. Antibody at 3 ug/mL. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Serpin I2 Antibody Summary

• Description: Novus Biologicals Goat Serpin I2 Antibody (NB100-1782) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Serpin I2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KGDWKQKFRKEDTQ corresponding to internal region according to NP_001012303.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_006208.1; NP_001012303.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SERPINI2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 ug/mL
  • Immunohistochemistry-Paraffin 3 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 40 kDa band observed in human pancreas lysates (calculated MW of 46.1 kDa band according to NP_006208.1 and 47.4 kDa band according to NP_001012303.1). IHC-P: Human pancreas show cytoplasmic staining of acinar cels and of rare cells in the islets of langerhans.
• Control:

  Human Pancreas Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Serpin I2 Antibody

• MEPI
• MEPIpancpin
• Myoepithelium-derived serine protease inhibitor
• Pancipin
• Pancpin
• Pancreas-specific protein TSA2004
• Peptidase inhibitor 14
• PI14
• PI-14
• PI14clade I (neuroserpin), member 2
• protease inhibitor 14
• serine (or cysteine) proteinase inhibitor, clade I (pancpin), member 2
• Serpin I2
• serpin peptidase inhibitor, clade I (pancpin), member 2
• SERPIN12

Background

The protein encoded by this gene is a member of the serine protease inhibitor (serpin) superfamily made up of proteins which play central roles in the regulation of a wide variety of physiological processes, including coagulation, fibrinolysis, development, malignancy and inflammation. The gene product may have a role in a growth-control, possibly growth-suppressing pathway and, when impaired, may be involved in pancreatic carcinogenesis. The protein is a member of the plasminogen activator inhibitor-1 family, a subset of the serpin superfamily whose members act as tissue-specific tPA inhibitors. Two alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin I2 Antibody (NB100-1782)(1)

Publication using NB100-1782

• Ozaki K, Nagata M, Suzuki M et al. Isolation and characterization of a novel human pancreas-specific gene, pancpin, that is down-regulated in pancreatic cancer cells. Genes Chromosomes Cancer 1998-07-01 [PMID: 9624529]

Bioinformatics

• Gene Symbol: SERPINI2
• Entrez:
  • 5276(Human)
  • 67931(Mouse)
  • 171149(Rat)
• Uniprot:
  • NP_006208.1(Human)