Serpin G1/C1 Inhibitor Overexpression Lysate (Native)

Images

 
Western Blot: C1 inhibitor Overexpression Lysate (Adult Normal) [NBL1-15855] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C1 inhibitor.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Serpin G1/C1 Inhibitor Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203767 and based on accession number NM_000062. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 (SERPING1), transcript variant 1, mRNA.
Gene
SERPING1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
52.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Serpin G1/C1 Inhibitor

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Serpin G1/C1 Inhibitor Overexpression Lysate (Native)

  • C1 Inhibitor
  • C1IN
  • C1INH
  • C1NH
  • HAE1
  • HAE2
  • Serpin G1
  • SERPING1 serpin peptidase inhibitor, clade G (C1 inhibitor), member 1

Background

This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its protein inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Serpin G1/C1 Inhibitor Lysate (NBL1-15855) (0)

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Bioinformatics

Gene Symbol SERPING1
Entrez