Human Serpin B5/Maspin ELISA Kit (Colorimetric)

ELISA: Human Serpin B5/Maspin ELISA Kit (Colorimetric) [NBP3-39789] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Standard Curve Range: 6.25 - 400 ng/mL (example only; lot dependent)
• Sensitivity: 2.32 ng/mL (example only; lot dependent)

Human Serpin B5/Maspin ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 6.25 - 400 ng/mL (example only; lot dependent)
• Sensitivity: 2.32 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SERPINB5

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Serpin B5/Maspin ELISA Kit (Colorimetric)

• Maspin
• Peptidase inhibitor 5
• PI5
• PI-5
• PI5protease inhibitor 5 (maspin)
• serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
• Serpin B5
• serpin peptidase inhibitor, clade B (ovalbumin), member 5

Background

Maspin is an extracellular matrix tumor supressor that blocks the growth, invasion, and metastatic properties of mammary and prostate tumors. Structurally Maspin is a serine protease inhibitor (Serpin), but it does not undergo the stressed to relaxed conformational transition characteristic of active serpins and exhibits no serine protease inhibitory activity. Maspin is also expressed by the corneal epithelium and regulates migration and adhesion of corneal stromal fibroblasts and myofibroblasts during corneal wound healing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Serpin B5/Maspin ELISA Kit (NBP3-39789). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SERPINB5