Serpin A9/Centerin Antibody [Unconjugated]

Serpin A9/Centerin was detected in immersion fixed paraffin-embedded sections of human lymphoma using Human Serpin A9/Centerin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5429) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Serpin A9/Centerin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Serpin A9
  Ala24-Thr417
  Accession # AAQ89063
• Specificity: Detects human Serpin A9 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SERPINA9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A9/Centerin Antibody [Unconjugated]

• Centerin
• GCET1
• Serpin A9
• SERPINA11
• SERPINA11b

Background

Serpin A9 (Serine proteinase inhibitor-clade A #9; also GCET1, Centerin and Serpin A11) is a 46 kDa, secreted member of the alpha 1-antitrypsin (clade A) subfamily, serpin superfamily of protease inhibitors. It is only produced by normal and lymphoma germinal center B cells. Its function is unknown, but it presumably acts as an inhibitor of a trypsin-like protease. Mature human Serpin A9 is presumably 394 amino acids (aa) in length. It contains one Serpin motif (aa 405-415). There are multiple splice variants associated with Serpin A9. There are alternate start sites at Met19, Met99 and Met150. A fourth isoform shows a two aa substitution for aa 369-435, while a fifth isoform shares this same substitution coupled with a 39 aa substitution for aa 1-75. A sixth variant shows a deletion of aa 91-190. Mature human Serpin A9 shares 67% aa identity with mouse Serpin A9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SERPINA9
• Uniprot:
  • AAQ89063()