Human Serotonin N-acetyltransferase ELISA Kit (Colorimetric)

ELISA: Human Serotonin N-acetyltransferase ELISA Kit (Colorimetric) [NBP3-27478] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Standard Curve Range: 1.56 - 100 U/mL (example only; lot dependent)
• Sensitivity: 0.6 U/mL (example only; lot dependent)

Human Serotonin N-acetyltransferase ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 1.56 - 100 U/mL (example only; lot dependent)
• Sensitivity: 0.6 U/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: AANAT

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Serotonin N-acetyltransferase ELISA Kit (Colorimetric)

• AA-NAT
• aralkylamine N-acetyltransferaseEC 2.3.1.87
• arylalkylamine N-acetyltransferase
• Serotonin acetylase
• SNATserotonin N-acetyltransferase

Background

Arylalkylamine N-Acetyltransferase (AANAT) is the main enzyme for melatonin synthesis. Hormonal melatonin has been implicated in sleep and circadian clock function, and acts as"the hormone of the night." Large changes in the abundance of AANAT are believed to be responsible for the large day/night rhythms in melatonin synthesis in the pineal gland and retina. Most research shows that AANAT activity is regulated by controlling the steady-state levels of the protein. Recent research, however, suggests that another mechanism may also play a role in controlling melatonin production without altering AANAT protein and activity. This mechanism may explain the sudden physiological increase in circulating melatonin observed at the onset of the dark period in humans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Serotonin N-acetyltransferase ELISA Kit (NBP3-27478). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: AANAT